DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral
DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral
DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral
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viii<br />
Table of contents<br />
Acknowledgements<br />
i<br />
Abstract<br />
iii<br />
Resumo<br />
v<br />
Table of contents<br />
viii<br />
List of figures<br />
xi<br />
List of tables<br />
List of abbreviations<br />
Chapter I 1<br />
I – Introduction 3<br />
I.1. Mesenchymal stem cells (MSCs) 3<br />
I.1.1 - Sources of MSCs 3<br />
I.1.2 -In vitro characterization of MSCs 4<br />
I.1.2.1 - Immunophenotype 5<br />
I.1.2.2 - CFU-F and proliferation capacity 5<br />
I.1.2.3 -Multilineage differentiation capacity 5<br />
I.2. - Mechanotransduction and its implications in cellular fate 6<br />
I.2.1 -Mechanotransduction and mechanosensors 9<br />
I.2.2 -Extracellular matrix and integrin-based mechanotransduction<br />
Mechanisms 11<br />
I.2.2.1 -Mechanisms of Rigidity Sensing 11<br />
I.2.3 -Mechanotransduction and Stem Cells 15<br />
I.2.3.1 - Mechanotransduction and MSCs differentiation 15<br />
I.2.3.1.1-Stiffness effect on Neuronal and Glial<br />
differentiation 19<br />
I.2.3.2-Manipulation and measurement of cellular forces<br />
in MSCs 21<br />
I.3 - Project rationale and experimental approach 24<br />
I.3.1 -Typical MSCs Isolation 24<br />
I.3.2 -Durotaxis, Tissue Elasticity and MSCs “memory” 24<br />
I.3.3 - Effects of the stiffness on the MSCs stemness genes 27
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