DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

20
(GFAP) (is a class-III intermediate filament which is the main constituent of
intermediate filaments in astrocytes and it serves as a cell specific marker that
distinguishes differentiated astrocytes from other glial cells during the development of
the central nervous system)(Eng et al., 2000); CNPase (is a myelin-associated enzyme
that makes up 4% of total central nervous system myelin protein and it is expressed
exclusively by oligodendrocytes and Schwann cells) (Scherer et al., 1994); MAP2
(encodes a protein that belongs to the microtubule-associated protein family which is
thought to be involved in microtubule assembly, it is an essential step in neurogenesis
and is commonly recognized as a neuronal mid/late marker) (Dehmelt et al., 2004) and
NF–H (for neuronal late marker, is a phosphorylated cytoskeletal intermediate filament
protein expressed in neurons) (Black et al., 1988).
Figure 9 - Characterization of neural-like lineage differentiation of hMSCs in 3-D scaffolds. The
graphic represents qRT-PCR results for representative neural lineage specific genes. D7= 7 days. D14 =
14 days. EDC_0.1% = ≈1kPa. EDC_2.0% = ≈10kPa (n =3,*P < 0.1, **P < 0.05) (Adapted from Her et al.,
2013).