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DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

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xvii<br />

List of tables<br />

Table I<br />

- Composition of the hydrogels solutions - volume added of each<br />

reagent (µL) per one milliliter of solution.<br />

Table II - Mean ± standard deviation (SD) of the Young’s modulus (E) (amount of<br />

force per unit of area needed to deform the material by a given fractional amount<br />

without any permanent deformation) calculated from the shear modulus measured at<br />

1Hz, according to the formula E= 2G’(1+ν), where G’ is the shear storage modulus<br />

measured by the rheometer and ν is the Poisson ratio, assumed to be 0.5 (Moore<br />

2010). Values represent results of measurement of three independent hydrogels (n=3).<br />

* Hydrogels previously characterized in the laboratory (Lourenço, 2012).<br />

Table III - Mean, Median and Coefficient of variation of the Fluorescence values<br />

obtained by flow cytometry for the positive MSCs markers (CD49e, CD73, CD13 and<br />

CD90) for cells isolated and cultured on PA hydrogels and from TCPs (n=1).

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