DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

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Figure 17| Colonies of hMSCs isolated in different PA hydrogels from UCM-WJ
fragments after 1 week and 2 weeks of fragments plating in the hydrogels with just
COL-1 or with COL-1 and FN (n=2). Bar corresponds to 200µm.
Figure 18| Immunophenotype of UCM-MSCs. The Y axis is the cell density. The X axis is
a logarithmic scale of fluorescence. Cells were detached, labelled with antibodies
against the indicated antigens and analyzed by flow cytometry. Cells were positive for
CD49e, CD73, CD13 and CD90 (red and green lines) when compared with unlabeled
MSCs (dark gray and light gray) for the 2 conditions, both from the PA hydrogels and
the Plastic/TCPs (n=1).
Figure 19| Immunophenotype of UCM-MSCs. The Y axis is the cell density. The X axis is
a logarithmic scale of fluorescence. Cells were detached, labelled with antibodies
against the indicated antigens and analyzed by flow cytometry. Cells were negative for
CD11b, HLA-DR, CD45 (red and green lines) when compared with unlabelled MSCs
(dark gray and light gray) for the 2 conditions, both from the hydrogels and of the
Plastic/TCPs (n=1).
Figure 20| hMSCs cultured on TCPs (Plastic) and PA hydrogels coated with COL-1 for 7
days after being treated with mitomycin C to inhibit proliferation. Cells were stained
with anti-B-III tubulin (red), anti-Nestin antibodies (green) and DAPI (blue). This
experiment was performed once (n=1). Bar corresponds to 400µm.
Figure 21| hMSCs cultured on TCPs (Plastic) and PA hydrogels coated with COL-1 for 7
days after being treated with mitomycin C to inhibit proliferation. Cells were stained
with anti-GFAP (red), anti-O4 antibodies (green) and DAPI (blue). This experiment was
performed once (n=1). Bar corresponds to 400µm.
Figure 22| hMSCs cultured on TCP (Plastic) and PA hydrogels coated with COL-1 for 7
days after being treated with mitomycin C to inhibit proliferation. Cells on the 7kPa
hydrogels and 1kPa hydrogels were isolated on 12 kPa hydrogels as mentioned in III.4.