European Society of Mycobacteriology - Instituto Nacional de Saúde ...

PP-45
Resistance, MDR and XDR of M. tuberculosis in Spain in the last years.
P. Ruiz, M. Causse, F.J. Zerolo, J. Gutierrez, M. Casal
Mycobacteria Reference Center. Department of Microbiology. Faculty of Medicine. HospitL “Reina Sofía” . Córdoba. Spain.
Tuberculosis is among the leading causes of death worldwide. The World Health Organization (WHO) estimates that
32% of the world population is infected with Mycobacterium tuberculosis . The resistance to antituberculous drugs is a big
problem to the control of the illness . The emergence of multidrug-resistant strains (MDR), extensively drug-resistant
(XDR) and extreme drug-resistant (XXDR) strains, is a global problem that has made a considerable alarm. There is an
increasing demand to determinate in vitro susceptibilities of clinical isolates to antimicrobial agents other than those
considered primary drugs.
The purpose of this study, was determinate the resistance, MDR and XDR strains in our Reference Center in the four last years.
Material and method
We are studied 624 samples from patients suspects of tuberculosis. 355 strains of M. tuberculosis were isolates , in
BACTEC MGIT 960 and Lowenstein -Jensen medium and identified using Accuprobe and GENOTYPE MYCOBACTERIA.
The susceptibility testing was made for primary drugs and: Amikacin (AK) 1.0 µg/ml; Kanamycin (K) 1 µg/ml; Capreomycin
(CM) 2.5 µg/ml; Ethionamyde (ETH) 5.0 µg/ml; Ofloxacin (OF) 2.0 µg/ml; Ciprofloxacin (CI) 2 µg/ml ; Moxifloxacin (MX)
2 µg/ml; Levofloxacin (LE) 4µg/ml; Rifabutin (Rb) 0.5 µg/ml ;Rifapentine 5 µg/ml and Linezolid (Lz) 1.0 µg/ml. The Bactec
MGIT technique with standart protocol was strictly followed as recommended,
Results
From 624 samples, in 355 were isolated M. tuberculosis and 55 (15,49 %) of theme were resistant to some of the antimicrobial
agents studied. The resistance to streptomycin was 3´38 %, to rifampin 7,88%. ethambutol 1,12 %, isoniazid 11.26
% and pyrazinamide 1,97%. A 7,6 % of strains were resistant to some of the second line drugs.The MDR was 5,6 % and
the XDR 1,4 %. No XXDR were isolated.
Conclusion
The resistance to secondary drugs made necessary the in vitro studies. This method, BACTEC MGIT 960 is a reliable and
rapid method to determinate the secondary drugs.
European Society of Mycobacteriology | 30 th Annual Congress | July 2009 | Porto - Portugal
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