European Society of Mycobacteriology - Instituto Nacional de Saúde ...

SPOLIGOTYPING OF Mycobacterium tuberculosis ISOLATED FROM
PATIENTS OF CLEMENTE FERREIRA AMBULATORY IN SÃO PAULO, SP – BRAZIL
PP-17
Mello, Fernado Augusto Fiuza 1 , Albarral, Maria Idemar Pedrosa 1 , Mendes, Natália Helena 2 , Pandolfi, José Rodrigo
Cláudio 2 , Santos, Adolfo Carlos Barreto 2 , Almeida, Elisabete Aparecida 1 , Cardoso, Rosilene Fressatti 3 , Leite, Clarice
Queico Fujimura 2
1 - Clemente Ferreira Institute
2 - São Paulo State University
3 - Maringá State University
The molecular epidemiology study using different techniques revolutionized the understanding of the epidemiology
of tuberculosis allowing comparison between strains of Mycobacterium tuberculosis and tracking the movement of
individual strains. This project aims to use the technique of molecular epidemiology, Spoligotyping, trying to understand
more about the phenomenon of transmission in patients with pulmonary tuberculosis treated at Clemente Ferreira
Ambulatory (ambulatory of reference for the treatment of tuberculosis) in São Paulo city, from August 2006 to July 2008.
The clinical isolates were re-identified by molecular technique (PCR and PRA), and the strains identified as M. tuberculosis
conducted by the genotyping technique of Spoligotyping. From 102 isolates, the technique of IS 6110-PCR confirmed
the identification of M. tuberculosis in 96 clinical isolates and the PRA in 99, the remaining 3, 2 isolates identified as M.
avium subtype 2 and 1 unidentified mycobacteria. The results showed that 3 clinical isolates of M. tuberculosis had not
the IS6110 insertion sequence specific of M. tuberculosis, as well as 3 isolates identified in the clinic as M. tuberculosis
by molecular techniques were atypical mycobacteria. Of 96 isolates confirmed as M. tuberculosis, were analyzed by the
technique of Spoligotyping, a total of 89 isolates, which revealed the presence of 21 strains (23.6%) with spoligotipes not
yet described in the data base world (spolDB4) and 68 (76.4%) of isolates involved in 7 different families, containing 2 to
30 isolates. The most frequent was T family with 30 isolates), followed by LAM (with 20 isolates) and Haarlem (with 10
isolates), which together accounted about 67.4% of all isolates. Were also identified 4 genotypes of the Beijing family, all
simultaneously resistant to isoniazid and rifampicin and / or more drugs.
European Society of Mycobacteriology | 30 th Annual Congress | July 2009 | Porto - Portugal
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