European Society of Mycobacteriology - Instituto Nacional de Saúde ...

PP-16
GENOTYPING OF Mycobacterium tuberculosis
IN NORTHWEST OF PARANÁ STATE OF BRAZIL
Leite, Clarice Queico Fujimura 1 , Nogutia, Erika N 2 , Malaspina, Ana Carolina 1 , Santos,
Adolfo Carlos Barreto 1 , Hirata, Rosáro DC 3 , Hirata, Mário H 3 , Cardoso, Rosilene Fressatti 2
1 - São Paulo State University
2 - Maringá State University
3 - University of São Paulo
Introduction
Worldwide, tuberculosis (TB) remains the most frequent and important infectious disease causing morbidity and death.
The molecular epidemiology study using different techniques revolutionized the understanding of the epidemiology of
tuberculosis allowing comparison between strains of Mycobacterium tuberculosis and tracking the movements of individual
lines. We reported here the first insight about the genetic diversity of M. tuberculosis in northwest of Paraná State,
south of Brazil. This knowledge encourages additional prospective epidemiological study for evaluation of the Regional
Tuberculosis Control Plan in this setting.
Objectives
Provide information about the genetic diversity and prevalent genotype of Mycobacterium tuberculosis and compare the
usefully of two methodologies in epidemiological study of tuberculosis in low endemic area in south of Brazil. Material
and Methods: We used spoligotyping and MIRU-VNTR typing to genotype M. tuberculosis isolates.
Results
The 93 isolates analyzed by spoligotyping were divided into 36 different patterns and 25 were described in the SpolDB4.0
database. Latin American and Mediterranean, Haarlem and T family were responsible for 26.9%, 17.2% and 11.8%, of
tuberculosis cases respectively. From the 84 isolates analyzed by MIRU-VNTR typing, 58 showed unique pattern and 26
belonged to 9 clusters. The MIRU loci 40, 23, 10 and 16 were the most discriminatory. MIRU-VNTR and spoligotyping
combined showed 85.7% of discriminatory power (HGI=0.995).
Conclusions
Spoligotyping and MIRU-VNTR typing combined are useful tool for epidemiological study in this low endemic setting in
south of Brazil and tuberculosis predominantly develops through reactivation of latent infection.
88 ESM 2009