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GENOTYPING OF MONO AND MULTI-DRUG RESISTANCE TB IN SAUDI ARABIA PP-23 Sahal Al-Hajoj 1 , Bright Varghese 1 , Mais Herbawi 1 , Ruba Al-Omari 1 and Caroline Allix-Béguec 2 1 - King Faisal Specialist Hospital and Research Centre, Comparative Medicine Tuberculosis Research Unit Saudia Arabia 2 - Genoscreen A total of 150 isolates collected from different regions in Saudia Arabia were the subject of finger printing using GenoScreen MIRU-VNTR kit (do you know the timeframe?). Upon genotype the data were compared to the international MIRU- VNTRplus database (www. Miru-vntrplus.org). The data showed that Saudia Arabia harbors the following major clades EAI 13.07%, Haarlem 12.42%, TUR 13.07%, Beijing 12.42%, unknown12.42%, Dehli/CAS 7.84%, LAM 7.19%, Cameroon 6.54%, UgandaI 5.23%, S 3.92%, multiple matches1.96%, NEW-1 1.31%, URAL 0.65%, Ghana 0.65%, X, 0.65%, UgandaII, 0.65%. Indicate the clustering rate for this panel of 150 isolates. Ongoing transmission may be an indication to the need of improving TB control program. The unknown clades represent a considerable % (12.42%). These could represent unique endogenous clades. However, further analysis is needed to gain insight into the nature of the unknown clades. European Society of Mycobacteriology | 30 th Annual Congress | July 2009 | Porto - Portugal 95
PP-24 early detection of MDRtb by molecular tools in the control of drug resistant tuberculosis in portugal: a case of success Diana Machado 1 , Miguel Viveiros 1,2 , Liliana Rodrigues 1,3 , Isabel Couto 1,4 , Leonard Amaral 1,2,3 1 - Unit of Mycobacteriology, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT/UNL), Lisbon, Portugal 2 - COST ACTION BM0701 (ATENS) 3 - UPMM, IHMT/UNL, Lisbon, Portugal 4 - Centro de Recursos Microbiológicos (CREM), Faculdade de Ciências e Tecnologia, UNL, Caparica, Portugal Multidrug resistant tuberculosis (MDRTB) represents a threat to public health and a challenge to tuberculosis (TB) control programs. From 1994 to 1997, Portugal had an incidence of 48.3 new cases of TB per 100 000 inhabitants and an average of 22.7% of these cases were MDRTB, the highest in Western Europe. In an attempt to assist the National Health Authorities in the control of TB, we implemented in 2002 with the support of Fundação Calouste Gulbenkian, the “TB Fast-Track” program as part of the TB Task Force of Greater Lisbon, involving 12 Lisbon hospitals and based on the use of the BACTEC TM MGIT 960 system, coupled with the direct identification of M. tuberculosis complex (MTBC) and the detection of mutations in the rpoB gene, using the INNOLiPA Rif.TB Assay (LiPA) (Innogenetics). Because mutations in the rpoB result in resistance to rifampicin (RIF), and resistance to RIF is almost always accompanied by resistance to isoniazid (INH), this approach allowed us to identify the MDRTB patient within 24-48 hours. A full report confirming identification and antibiotic susceptibility (AST) of MTBC by conventional methods (BACTEC culture plus AST and Accuprobe ID) was issued within additional 12 days. From September 2002 to January 2006, the LiPA assay was directly applied to 630 acid fast positive respiratory specimens. The comparison between data from this assay and conventional methods revealed 84 discrepancies. The 11 false positive results corresponded to patients with therapy already established by the time of specimen sampling, whereas the 73 false negative resulted from inhibition of amplification. A total of 487 of the 600 MTBC positive isolates were susceptible to all 5 first-line anti-TB drugs. The frequency of MDRTB (resistant to at least INH plus RIF) was 10%. From the 63 MTBC resistant to RIF, 62 were detected by the LiPA as carrying mutations S531L (60 isolates), H526Y and D516V (1 isolate each). No mutation was detected by LiPA for one sample, repeatedly identified as resistant by AST. Detection of rpoB mutations proved to be a good surrogate marker for MDRTB, since only 2 out of 600 MTBC isolates were monoresistant to RIF. The early detection of active TB, particularly the detection of MDRTB, is essential for the success of any TB control program. The application of molecular techniques for the early identification of MDRTB assisted the National Health Authorities in the reduction of MDRTB rates in Lisbon to less than 8% (average 2003 to 2007). 96 ESM 2009
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ESM European Society of Mycobacteri
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Welcome Message Dear Colleagues, It
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Congress Organization EUROPEAN SOCI
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Program at a glance Sunday, July 5t
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Wednesday, July 8th 09h00 - 11h00 0
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Dr. Andre De Bock (BD) Extended Dru
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16h30 - 16h45 Santos R, Marques M,
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09h45 - 10h30 10h30 - 10h45 10h45 -
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Mello FAF, Albarral MIP, Mendes NH,
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Lima KVB, Lopes ML, Furlaneto IP, L
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Julián EG, Rodríguez-Güell E, de
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Abstracts of Guest Lectures (GL) Eu
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GL-2 THE BRAZILIAN EXPERIENCE CONTR
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GL-3 EVOLUTIONARY FORCES IN Mycobac
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GL-5 SURROUNDED BY MYCOBACTERIA Jos
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GL-7 A NOVEL DIAGNOSTIC TEST TO DIF
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GL-9 REGULATION OF Mycobacterium tu
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GL-11 DEVELOPMENT AND VALIDATION OF
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SCREENING OF MOLECULES WITH ANTI-TB
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GL-15 NEW, EASY-TO-USE TOOLS FOR QU
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OP-1 Mass Spectrometry for Molecula
Page 46 and 47: OP-3 IDENTIFICATION OF THE INSERTIO
Page 48 and 49: OP-5 PENITENTIARY POPULATION OF Myc
Page 50 and 51: op-7 Mycobacterium tuberculosis gen
Page 52 and 53: OP-9 LAM AND HIV: CORRELATION OR CO
Page 54 and 55: OP-11 Mycobacterium avium SUBSPECIE
Page 56 and 57: OP-13 THE SUNNY SIDE OF MYCOBACTERI
Page 58 and 59: OP-15 HOW WILD-TYPE MIC DISTRIBUTIO
Page 60 and 61: OP-17 Mycobacterium tuberculosis IS
Page 62 and 63: A REPORT ON NEW ADAPTED FORMS OF EX
Page 64 and 65: OP-21 PRESENCE OF ESAT-6 AND CFP-10
Page 66 and 67: OP-23 TUBERCULOSIS SOFTWARE IN A GE
Page 68 and 69: OP-24 Development of an automated c
Page 70 and 71: OP-26 THIORIDAZINE SHOWS PROMISING
Page 72: OP-28 MEMBRANE- BASED VERSUS MICROB
Page 76 and 77: PP-1 EVALUATION OF THE HIGH-THROUGH
Page 78 and 79: PP-3 ASSOCIATION BETWEEN BEIJING GE
Page 80 and 81: PP-6 SPOLIGOTYPE PATTERNS AND DRUG
Page 82 and 83: PP-8 Mycobacterium tuberculosis BEI
Page 84 and 85: PP-11 FITNESS STUDY OF THE RD RIO L
Page 86 and 87: PP-13 IMPORTANCE OF MOLECULAR TYPIN
Page 88 and 89: PP-15 MOLECULAR EPIDEMIOLOGY STUDY
Page 90 and 91: SPOLIGOTYPING OF Mycobacterium tube
Page 92 and 93: PP-19 MULTIPLY RECURRENT TUBERCULOS
Page 94 and 95: PP-21 MOLECULAR STUDY OF RECURRENT
Page 98 and 99: PP-25 Drug-resistance of Mycobacter
Page 100 and 101: PP-27 Mutational analysis of genes
Page 102 and 103: PP-29 CHARACTERISATION OF STREPTOMY
Page 104 and 105: PP-31 tuberculosis RESISTANCE in a
Page 106 and 107: PP-33 GENOTYPIC DETECTION OF ISONIA
Page 108 and 109: PP-35 Mycobacterium tuberculosis: 1
Page 110 and 111: PP-37 IN VITRO ACTIVITY OF OFLOXACI
Page 112 and 113: DETECTION OF EMBB GENE CODON 306 MU
Page 114 and 115: PP-41 Characterization of gidB gene
Page 116 and 117: PP-43 DESIGN OF A RAPID METHOD OF I
Page 118 and 119: PP-45 Resistance, MDR and XDR of M.
Page 120 and 121: PP-47 TYPING OF Mycobacterium avium
Page 122 and 123: PP-49 IS1245-RFLP Based Genetic Rel
Page 124 and 125: PP-51 Nontuberculous Mycobacteria,
Page 126 and 127: PP-53 EVALUATION OF HSP 65, TB ,SP
Page 128 and 129: IDENTIFICATION OF NONTUBERCULOUS MY
Page 130 and 131: PP-57 ISOLATION AND IDENTIFICATION
Page 132 and 133: PP-59 A CASE-REPORT OF Mycobacteriu
Page 134 and 135: PP-61 LABORATORY MICROBIOLOGY CONTR
Page 136 and 137: TEACHING OLD BONES NEW TRICKS; SING
Page 138 and 139: DIRECT IDENTIFICATION OF Mycobacter
Page 140 and 141: PP-67 FIRST EXPERIENCE WITH GENOTYP
Page 142 and 143: PP-69 EVALUATION OF A NEW REAL-TIME
Page 144 and 145: CLINICAL PERFORMANCE OF QUANTIFERON
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PP-73 QUANTIFERON ® -TB GOLD IN-TU
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REAL-TIME POLYMERASE CHAIN REACTION
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PP-77 DETECTION OF Mycobacterium tu
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PP-79 OSSEOUS TUBERCULOSIS AT AGE O
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PP-81 ROLE OF TNF-a GENE POLYMORPHI
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PP-83 VIRULENCE, IMMUNOGENICITY AND
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PP-85 ANALYSIS OF M. smegmatis MUTA
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PP-87 Mycobacterium tuberculosis Ar
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IMPLEMENTATION OF THE THIN LAYER AG
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PP-91 DIRECT DETECTION OF RIFAMPIN
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PP-93 CONTRIBUTION OF LABORATORY FA
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PP-95 EVALUATION OF CAPILIA (TAUNS)
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PP-97 DIRECT TESTING FOR MULTI DRUG
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PP-99 VARIOUS STRATEGIES TO DECONTA
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PP-101 COMPARISON OF RAPID COLORIME
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PP-103 NITRATE REDUCTASE ASSAY APPL
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PP-105 Implementation of liquid cul
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PP-107 SYNERGISTIC ACTIVITY OF TWO
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PP-109 PREVALENCE OF EFFLUX-MEDIATE
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PP-111 ANTI-MYCOBACTERIUM TUBERCULO
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PP-113 the human macrophage as a mo
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PP-115 Nosocomial TB in a laborator
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Levterova V Lezcano MA Lima EJC Lim
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