European Society of Mycobacteriology - Instituto Nacional de Saúde ...

PP-47
TYPING OF Mycobacterium avium subsp. avium FROM
DIFFERENT SOURCES USING PVUII–PSTI–IS901 RESTRICTION
FRAGMENT LENGTH POLYMORPHISM (RFLP) IN CROATIA
Spicic, Silvio 1 , Cvetnic, Zeljko 1 , Pate, Mateja 2 , Duvnjak, Sanja 1 , Zdelar-Tuk, Maja 1 , Racic, Ivana 1
1 - Croatian Veterinary Institut, Zagreb
2 - Veterinary Faculty Ljubljana, Ljubljana, Slovenia
A total of 9 M. avium subsp. avium strains isolated from bovines (N=1), pigs (N=2), wild boars (N=2) and poultry (N=4)
were genotyped by PvuII–PstI–IS901 restriction fragment length polymorphism (RFLP) analysis. The isolates were collected
in the period from 2001 to 2006. Revealed profiles were designated according to the nomenclature established
and used in the OIE reference laboratory for avian tuberculosis in Brno, Czech Republic. Digestion with restriction endonuclease
PvuII resulted in 3 PvuII RFLP profiles (F, Q and M), comprising 8-9 bands. Digestion with restriction endonuclease
PstI was successfully accomplished in 8 isolates demonstrating 4 different profiles of 11-13 bands. Among them, 3
were found to be new in the database (A31, A32 and A33). Combination of PvuII–PstI digestion revealed 4 RFLP profiles
(A29/F, A31/F, A32/F, A33/M). No epizootiological connection was found between the isolates expressing the predominant
profile (A29/F), found in pigs, wild boars and poultry. This is the first research in the field of genotyping M. avium subsp.
avium strains isolated from different animal species in Croatia.
European Society of Mycobacteriology | 30 th Annual Congress | July 2009 | Porto - Portugal
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