Annals of Warsaw University of Life Sciences – SGGW. Animal ...

26 J. Gajewska et al.
effect, e.g. L-carnitine used most often
in flocks of breeding poultry, improving
sperm concentration, hatchability and
affecting production results (Zhai, 2008;
Keralapurath et al., 2010; Shafey et al.,
2010). Application of 20 to 100 mg per
kg feed, L-carnitine in the nutrition of
poultry for slaughter allows for a reduction
of fat content in carcass (Rabie et al.,
1997a, b; Arslan et al., 2004). There are
however, no reports on the effects of L-
-carnitine on the composition of intestinal
microflora of broiler chickens therefore
Aminokarnifarm was used to because
(43.68% of L-carnitine) to determine
the composition of the microflora in the
small intestine of chickens.
MATERIALS AND METHODS
The experiment was conducted in poultry
farm of Experimental Station (RZD)
Wilanów-Obory on 640 broiler chickens
of COBB 500. One-day chicks were at
random allocated to 2 groups: control
and experimental (Aminokarnifarm), each
of them in four repetitions. The birds
were kept on the litter, according to the
standards for management of broilers, as
recommended by Cobb company. The
differentiating factor was the administration
of the group Aminokarnifarm
to water for the experimental group of
chickens.
Aminokarnifarm is a preparation
amino acid vitamin, which a combination
of vitamins and amino acids with taurine
(13.33%) and L-carnitine (43.68%) has
a very strong anabolic effects, improving
production results. The preparation
ensures the proper metabolism of fatty
acids and amino acids, sufficient production
of energy from ketones, regulation
of concentration of ammonia in the
blood, stimulation the immune system,
the process of respiration and active
transport of ATP at the cellular level.
Composition of the preparation has a significant
impact on the proper functioning
of all systems in the body, in particular
neurohormonal system, digestive, circulatory
and reproductive. The presence of
L-carnitine determines the capacity of
the body, muscle strength, cardiac activity,
in combination with the metabolism
of amino acids enhances the whole body
and neutralizes the negative effects of
various types of stress factors (Biofaktor
– producer information).
Aminokarnifarm was added in drinking
water in quantities of 100 g of the
preparation of 160 liters of water, rearing
on the following dates: 1–7 days, 21–28
days, 35–42 days. During the rearing
of chickens from the control group and
experimental (Aminokarnifarm) were fed
compound having the following nutritional
value: Starter from 1 to 14 day
(22.1% crude protein and 13.7 MJ of
EM). Grower from 15 to 35 day (20.4%
crude protein and 14.1 MJ of EM).
Finisher from 36 to 42 day (19.1%
crude protein and 14.3 MJ EM, without
coccidiostatic). On 42 day, from 9 males
and 9 females, parts of intestinal were taken
after slaughter. The aim was to determinations
quantitative and qualitative factors
of intestinal microflora in small intestine
content of chicken broilers.
Quantitative determination of small
intestine content, received from two
feeding groups, were done by standat
Koch’s plate method, with surface sowing
(Salyers, Whitt, 2003).
The following microbiological rates
were determined: