Annals of Warsaw University of Life Sciences – SGGW. Animal ...

42 J. Gruszczyńska, M. Łukasiewicz
in terms of the MCW0145 sequence,
whereas Wardęcka et al. (2002) reported
3 alleles in each ivestigated breed (156bp
and 206bp both for Green-Legged and
Rhode Island Red–RIR, 196bp–typical
of Green-Legged Partrigenous, 202bp–
–typical of RIR). In broiler chickens,
Weissmann et al. (1998) showed 4 alleles
in locus ADL0306. The length of alleles
noted in Ayam Cemani hens was similar
to that reported for other breeds (Tab. 3).
However, in terms of the selected microsatellite
sequence, some new alleles
have been found in the examined population
of hens, i.e. in locus MCW0145
these were 214bp and 218bp alleles and
in locus ADL0306 these were 119bp and
125bp alleles (Tab. 3).
The frequency of alleles of the microsatellite
sequences determined in the
studied population enabled determining
indices of observed (H O ) and expected
(H E ) heterozygosity as well as the polymorphic
index (PIC). Values of the H E
ranged from 0.41 to 0.64, and these of
the PIC index were slightly lower (Tab.
4). Other authors reported higher values
(ca. 0.7) of these indices (Lujiang et al.,
2006; Tadano et al., 2007b). The highest
percentage (81%) of homozygotes was
reported in the terms of the MCW0184
sequence, while the lowest (4%) in terms
TABLE 3. Characteristics of 5 selected microsatellite sequences in Ayam Cemani and other chicken
breeds
Microsatellite
sequence
Sequence
localization
in chromosome
of
domestic
hen
Ayam
Cemani in
this study
length of
alleles (bp)
number
of alleles
8 a)
3 i)
3 i)
8 g)
7 h)
4 f)
4 f)
2 d)
length of alleles
(bp)
Other authors
2 k) 186; 195
164–212
156; 202; 206
MCW0145 1
156; 196; 206
206;
181–209
212;
189–211
214;
164; 192; 203; 207
218
192; 203; 205; 210
186; 195
MCW0184 2 240; 246 6 b) 240–293 Broiler
MCW0210 5
156;166; 4 b) 152–186
Broiler
168 4 j) 148; 152; 156; 160 Broiler
LEI0071 1
7
280;
281–330
Broiler
8
290;
214; 224; 230; 277; Broiler
308; 310; 318; 327
300
1 c) 263
ISA lines
ADL0306 3
119;121; 4 j) 110; 113; 117; 121
125 1 e) 127
line or breed or type
of chicken
Broiler
Rhode Island Red
Green-Legged Partrigenous
Japanese long-tailed
Japanese long-tailed
Broiler
Layer
Guangxi Three-yellow
Qingyuan Partridge
Broiler
NicholasCommercial line
a)
Crooijmans et al. (1996); b) Crooijmans et al. (1997); c) Gibbs et al. (1997); d) Li et al. (2009); e) Reed
et al. (2000); f) Rosario et al. (2009); g) Tadano et al. (2007a); h) Tadano et al. (2007b); i) Wardęcka et al.
(2002); j) Weissmann et al. (1998); k) Zou et al. (2010)