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Spectroscopy in a Suitcase - Royal Society of Chemistry

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ULTRAVIOLET - VISIBLE SPECTROSCOPY (UV)<br />

EXERCISE 4 - INVESTIGATING TRANSITION METAL COMPLEXES 5<br />

Investigation 2<br />

Beer Lambert Law<br />

CONCENTRATION (mol dm -3 )<br />

ABSORBANCE<br />

4.0 x 10 -4 0.775<br />

3.2 x 10 -4 0.639<br />

2.4 x 10 -4 0.516<br />

1.6 x 10 -4 0.336<br />

0.8 x 10 -4 0.194<br />

Unknown 0.395<br />

Beer-Lambert Calibration Plot – Potassium Permanganate Solution<br />

0.900<br />

0.800<br />

0.700<br />

0.600<br />

Absorbance<br />

0.500<br />

0.400<br />

0.300<br />

0.200<br />

0.100<br />

0.000<br />

0.0E+00 5.0E-05 1.0E-04 1.5E-04 2.0E-04 2.5E-04 3.0E-04 3.5E-04 4.0E-04 4.5E-04<br />

Concentration (mol dm -3 )<br />

Answers<br />

1.The plot <strong>of</strong> absorbance versus concentration is a straight l<strong>in</strong>e graph.<br />

2.From the graph the solution is 2.1 x 10 -4 mol dm -3 the unknown solution prepared was 2.0 x 10 -4 mol dm -3<br />

Copyright © 2009 <strong>Royal</strong> <strong>Society</strong> <strong>of</strong> <strong>Chemistry</strong> www.rsc.org

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