Golden Alga Workshop Summary Report - Texas Parks & Wildlife ...

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Relation between mortality of Oxyrrhys at different cells densities and P- deficient P. parvum cells (20000 . ml -1 ) 100 Oxyrrhis mortality (% of control) 80 60 40 20 0 1 2 3 4 5 Number of Oxyrrhis (220 to 81000 cells . ml -1 ) PWD RP T3200-1203
Ingestion of Oxyrrhis after 2 and 6 days incubation with increasing Rhodomas and Prymnesium densities Ingestion (algae grazer -1 d -1 ) 2 days 15 10 5 0 0 10 20 30 40 50 6 days 6 5 4 3 2 1 0 0 10 20 30 40 50 Initial Rhodomonas ( ) or Prymnesium ( ) cell densities (x10 3 ml -1 ) 7 PWD RP T3200-1203
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Golden Alga (Prymnesium parvum) Wor
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Golden Alga Workshop Agenda October
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Golden Alga Workshop Table of Conte
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Golden Alga Workshop Background Inf
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The TPWD “GOLD” Team Technical
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While There Is Much We Do Not Know
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Detection Early Simple Basic Resear
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Historical Review of Golden Alga (P
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Prymnesium parvum PWD RP T3200-1203
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Prymnesium parvum in Texas: 28 eve
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Five Texas River Basins Impacted C
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Number and Value of Fish Killed 198
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1 1 5 5 6 6 14 13 15 12 14 13 15 12
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Month Golden Alga Fish Kills Began
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Number of Fish Killed Brazos Basin
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We Want to Solve the Problem Factor
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What We Have Done - continued Repor
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Special Thanks To The Following: We
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Overview of Texas Hatchery Manageme
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Dundee State Fish Hatchery • Febr
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Dundee State Fish Hatchery • Caus
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TPWD Inland Fisheries P. parvum Tas
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Monitoring P. parvum densities •
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Monitoring Toxin Levels • Bioassa
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Monitoring of P. parvum • Needs -
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Physical Treatments Χ Sonication (
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Χ X Chemical Treatments Hydrogen p
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Treatments - Chemical • Ammonium
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Treatments - Chemical • Cutrine
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Achievements • Development of hat
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Phylogeny, life history, autecology
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Overview • morphology - what it l
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Morphology of P. parvum haptonema f
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Prymnesium species Species Habitat
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Haplo-diploid life cycle P. parvum
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Life cycle: mating experiment 2n +
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Possible life cycle for P. parvum H
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Optimum and tolerance for growth Pa
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Mixotrophy • P. parvum can ingest
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Toxins • proteolipids (Ulizur & S
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Occurrence of harmful Typical habit
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What can be done? • Establish a m
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Prymnesium parvum: An overview and
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Presently recognized Prymnesium spe
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Bloom History • Within a decade o
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PWD RP T3200-1203
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Dying Shad - Texas - courtesy of C.
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PWD RP T3200-1203
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Dead fish at a dam site in Texas -
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Fish showing hemmoragic areas from
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Toxins: (Lethal Cocktail) There is
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How easily can they be identified f
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PWD RP T3200-1203 Body Scales of P.
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Fluorescent labeled P. parvum cell
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Priorities: Cells: • accurate and
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Analysis of Prymnesium parvum bloom
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Overview • Questions Identified b
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Approach - proposed studies • Syn
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Lake Whitney Stations Site P11 Site
Page 123 and 124: 80000 Lake Whitney 2003 P. parvum a
Page 125 and 126: Feb-April 2003 Lake Whitney: surfac
Page 127 and 128: Lake Bioassay Methods • Acclimate
Page 129 and 130: Lake Whitney Bioassay Sites Site P1
Page 131 and 132: Lake Whitney Nutrient Bioassay 4/29
Page 133 and 134: Original Questions • What factors
Page 135 and 136: Next steps? • Continue paired-res
Page 137 and 138: DY III Media Stock Addtions Nutrien
Page 139 and 140: Kill your enemies and eat them: the
Page 141 and 142: 5 µm Killed salmons in aquaculture
Page 143 and 144: When will the first dead bathing gu
Page 145 and 146: PWD RP T3200-1203
Page 147 and 148: Toxicity in Chrysochromulina polyle
Page 149 and 150: HE 50 (10 6 cells ml -1 ) Prymnesiu
Page 151 and 152: Effect of increasing P-deficent P.
Page 153 and 154: Ichthyotoxic species Prymnesin-2 H
Page 155 and 156: ☺ Before PWD RP T3200-1203
Page 157 and 158: Effect of Prymnesium parvum filtrat
Page 159 and 160: Skovgaard and Hansen, 2003, L & O,
Page 161 and 162: Ciliates Flagellates Diatoms cell l
Page 163 and 164: Low Prymnesium parvum cell numbers
Page 165 and 166: 6 Bacterivory in P. parvum Ingested
Page 167 and 168: P. parvum phagotrophy (prey ingesti
Page 169 and 170: % P. parvum feeding cells and lysed
Page 171 and 172: Skovgaard and Hansen, 2003, L & O,
Page 173: Oxyrrhis marina cell lysis in the p
Page 177 and 178: P-deficient P. parvum ingesting blo
Page 179 and 180: Conclusions •Toxicity is the key
Page 181 and 182: C. helgolandicus pellets •Picture
Page 183 and 184: Laboratory Studies on Prymnesium -
Page 185 and 186: News Release • May 28, 2002 - mas
Page 187 and 188: Fish Kill at Prewitt Reservoir PWD
Page 189 and 190: Pelicans putative of transport agen
Page 191 and 192: Objectives of this Presentation •
Page 193 and 194: Prymnesium Strains in Culture • U
Page 195 and 196: Structure of Prymnesium PWD RP T320
Page 197 and 198: PWD RP T3200-1203
Page 199 and 200: Scales Haptonema PWD RP T3200-1203
Page 201 and 202: Cyst Formation • A variety of con
Page 203 and 204: Growth Observations 0n Prymnesium s
Page 205 and 206: Prymnesium Twin Buttes Lake, Wyomin
Page 207 and 208: Hemolytic Bioassay Procedures • 5
Page 209 and 210: Hemolysis Bioassay • Alga Medium
Page 211 and 212: Mixotrophy Studies • Several phyt
Page 213 and 214: Chloroplast Endoplasmic Reticulum N
Page 215 and 216: Adaptive Advantage of Chloroplast E
Page 217 and 218: 10 µm Kathablepharis sp. Courtesy
Page 219 and 220: Chrysochromulina parva Long, uncoil
Page 221 and 222: Ingested Chrysochromulina & some ba
Page 223 and 224: Return to Prewitt • Prymnesium pa
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Prewitt Reservoir Fact • Five day
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Prewitt Reservoir Explanation • P
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Prewitt Mystery Solved? • Campylo
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Future Prymnesium Problems in Wyomi
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Rapid Tests for the Detection of Pr
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Why rRNA probes? * universally foun
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PWD RP T3200-1203
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EUK 1209R CHLO 02 PRYM 02 PELA 01 P
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Change from PFA to ETOH Saline Chan
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Analysis of Alexandrium strains fro
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PWD RP T3200-1203
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Harmful Algal Blooms Noctiluca Kare
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PWD RP T3200-1203 Coccolithus pelag
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Application & detection methods for
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Conclusions • Specific probes cou
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1. ChemScanRDI, a laser based syste
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ChemScanRDI combines fluorescent ce
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Electrochemical detection of toxic
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Electrochemical Detection of rRNA f
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Comparison of cell counts with elec
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Long term stability of treated sens
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Development of DNA-microarrays for
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Scheme of a DNA-Chip Experiment Flu
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Monitoring Phytoplankton compositio
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EUK 1209 CHLO01 Localization of the
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Identification of Phytoplankton on
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DNA CHIP Clone librairy Dec 2000 1
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Research Needs • Monitoring of to
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Rapid Tests for the Detection of Ha
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abs. units 1.0 0.8 0.6 0.4 0.2 y =
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Prymnesium parvumRL10 % lysis 100 8
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PWD RP T3200-1203 Allelochemical ef
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Controlling Harmful Algal Blooms Do
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Management of harmful algae • Pre
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Chemical control of freshwater alga
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•This (small) reservoir had a lon
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Chemical flocculants - Phosphorus C
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PWD RP T3200-1203 from: River Scien
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Biological Control - Viruses Aureoc
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Viruses for HAB species Target spec
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Biological Control - Bacterial path
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Biological Control - Grazers Target
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Clay control of HAB species clay mi
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Variable removal ability of domesti
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PWD RP T3200-1203 Source: Sengco et
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Flume studies, WHOI PWD RP T3200-12
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Brevetoxin analysis cell concentrat
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Impacts - Benthic fauna Archambault
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Future directions: Cell removal, se
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When the cells reached exponential
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Conclusions Kalmar Experiments Phos
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Experiments at Woods Hole (in colla
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Recent work in Kalmar (data from J.
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Conclusions - control of Prymnesium
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A review of fish-killing microalgae
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Toxic/harmful microalgae • dinofl
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PWD RP T3200-1203 Fish kills
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Ichthyotoxic species • Karenia br
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FMRI,FWC Exposure routes • gills
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Gymnodinium pulchellum (brevetoxins
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Reef fish disease - Caribbean, Flor
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Diatoms • physical damage to gill
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Aquaculture in Israel and Prymnesiu
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Prymnesium parvum from Israel PWD R
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Prymnesium parvum blooms in Israel
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From Sarig 1971 Prymnesium parvum t
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From Sarig 1971 Mode of toxin actio
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Fish bioassay • dependence of tox
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Relationship # toxin concentration
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From Sarig 1971 Management of Prymn
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Management strategies • prevent b
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How to Use the Past to Plan for the
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How can studies on other harmful al
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Task Force report identified 7 HAB
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A 5-year federal, state, academic,
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ECONOMIC IMPACT In the 1970s, two r
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What is Needed Based on What is Kno
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Golden Alga Workshop Notes: Present
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facilities was a problem in North C
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Korean fisheries are a $1 billion i
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Friday October 24, 2003 Panel Discu
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• Design a sampling program with
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What are your recommendations for i
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• Rapid progress is made where th
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• Maintain a Golden Alga web site
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ChemScanRDI (a laser based system t
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• DNA-microarrays for monitoring
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• Reliable economic statistics fo
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• Be proactive; get messages out
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Golden Alga Workshop Attendees Firs
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Golden Alga Workshop Attendees, con
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Literature Review of the Microalga
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Oued Mellah Reservoir in Morocco oc
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the metal ions Fe, Zn, Mo, Cu or Co
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with the ability to utilize organic
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Figure 1. Prymnesin-1 (1) and Prymn
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Temperature Shilo and Ashner (1953)
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Glycerol Glycerol was found to incr
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since it seems that nitrogen and ph
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(Haptophyta) in semi-continuous cul
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Ulitzer, S. and M. Shilo. 1966. Mod
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