Golden Alga Workshop Summary Report - Texas Parks & Wildlife ...

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Double Staining of Cells to differentiate cells hierarchically 250 Relative Intensity 200 150 100 50 0 0 5 10 15 20 25 Pr y mGlA FLUOS Distance[µm] NoProbe Euk1209 CY5 The cells were hybridised with the universal eukaryotic probe (labelled blue) and the genus specific probe for Prymnesium PrymGl01A (labelled green). Mixture of E.huxleyi and Prymnesium PWD RP T3200-1203 parvum
Analysis of Alexandrium strains from European waters using hierarchical probes EUK1209 DINO B ATAM01 ATNA02 ATWE03 ATME04 ATME05 ATME06 Alex. tamarense (English Channel) Alex. cf. tamarense (Italy) PWD RP T3200-1203
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Golden Alga (Prymnesium parvum) Wor
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Golden Alga Workshop Agenda October
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Golden Alga Workshop Table of Conte
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Golden Alga Workshop Background Inf
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The TPWD “GOLD” Team Technical
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While There Is Much We Do Not Know
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Detection Early Simple Basic Resear
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Historical Review of Golden Alga (P
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Prymnesium parvum PWD RP T3200-1203
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Prymnesium parvum in Texas: 28 eve
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Five Texas River Basins Impacted C
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Number and Value of Fish Killed 198
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1 1 5 5 6 6 14 13 15 12 14 13 15 12
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Month Golden Alga Fish Kills Began
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Number of Fish Killed Brazos Basin
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We Want to Solve the Problem Factor
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What We Have Done - continued Repor
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Special Thanks To The Following: We
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Overview of Texas Hatchery Manageme
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Dundee State Fish Hatchery • Febr
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Dundee State Fish Hatchery • Caus
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TPWD Inland Fisheries P. parvum Tas
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Monitoring P. parvum densities •
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Monitoring Toxin Levels • Bioassa
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Monitoring of P. parvum • Needs -
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Physical Treatments Χ Sonication (
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Χ X Chemical Treatments Hydrogen p
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Treatments - Chemical • Ammonium
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Treatments - Chemical • Cutrine
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Achievements • Development of hat
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Phylogeny, life history, autecology
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Overview • morphology - what it l
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Morphology of P. parvum haptonema f
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Prymnesium species Species Habitat
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Haplo-diploid life cycle P. parvum
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Life cycle: mating experiment 2n +
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Possible life cycle for P. parvum H
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Optimum and tolerance for growth Pa
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Mixotrophy • P. parvum can ingest
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Toxins • proteolipids (Ulizur & S
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Occurrence of harmful Typical habit
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What can be done? • Establish a m
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Prymnesium parvum: An overview and
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Presently recognized Prymnesium spe
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Bloom History • Within a decade o
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PWD RP T3200-1203
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Dying Shad - Texas - courtesy of C.
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PWD RP T3200-1203
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Dead fish at a dam site in Texas -
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Fish showing hemmoragic areas from
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Toxins: (Lethal Cocktail) There is
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How easily can they be identified f
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PWD RP T3200-1203 Body Scales of P.
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Fluorescent labeled P. parvum cell
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Priorities: Cells: • accurate and
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Analysis of Prymnesium parvum bloom
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Overview • Questions Identified b
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Approach - proposed studies • Syn
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Lake Whitney Stations Site P11 Site
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80000 Lake Whitney 2003 P. parvum a
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Feb-April 2003 Lake Whitney: surfac
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Lake Bioassay Methods • Acclimate
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Lake Whitney Bioassay Sites Site P1
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Lake Whitney Nutrient Bioassay 4/29
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Original Questions • What factors
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Next steps? • Continue paired-res
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DY III Media Stock Addtions Nutrien
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Kill your enemies and eat them: the
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5 µm Killed salmons in aquaculture
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When will the first dead bathing gu
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PWD RP T3200-1203
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Toxicity in Chrysochromulina polyle
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HE 50 (10 6 cells ml -1 ) Prymnesiu
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Effect of increasing P-deficent P.
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Ichthyotoxic species Prymnesin-2 H
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☺ Before PWD RP T3200-1203
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Effect of Prymnesium parvum filtrat
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Skovgaard and Hansen, 2003, L & O,
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Ciliates Flagellates Diatoms cell l
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Low Prymnesium parvum cell numbers
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6 Bacterivory in P. parvum Ingested
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P. parvum phagotrophy (prey ingesti
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% P. parvum feeding cells and lysed
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Skovgaard and Hansen, 2003, L & O,
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Oxyrrhis marina cell lysis in the p
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Ingestion of Oxyrrhis after 2 and 6
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P-deficient P. parvum ingesting blo
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Conclusions •Toxicity is the key
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C. helgolandicus pellets •Picture
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Laboratory Studies on Prymnesium -
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News Release • May 28, 2002 - mas
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Fish Kill at Prewitt Reservoir PWD
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Pelicans putative of transport agen
Page 191 and 192: Objectives of this Presentation •
Page 193 and 194: Prymnesium Strains in Culture • U
Page 195 and 196: Structure of Prymnesium PWD RP T320
Page 197 and 198: PWD RP T3200-1203
Page 199 and 200: Scales Haptonema PWD RP T3200-1203
Page 201 and 202: Cyst Formation • A variety of con
Page 203 and 204: Growth Observations 0n Prymnesium s
Page 205 and 206: Prymnesium Twin Buttes Lake, Wyomin
Page 207 and 208: Hemolytic Bioassay Procedures • 5
Page 209 and 210: Hemolysis Bioassay • Alga Medium
Page 211 and 212: Mixotrophy Studies • Several phyt
Page 213 and 214: Chloroplast Endoplasmic Reticulum N
Page 215 and 216: Adaptive Advantage of Chloroplast E
Page 217 and 218: 10 µm Kathablepharis sp. Courtesy
Page 219 and 220: Chrysochromulina parva Long, uncoil
Page 221 and 222: Ingested Chrysochromulina & some ba
Page 223 and 224: Return to Prewitt • Prymnesium pa
Page 225 and 226: Prewitt Reservoir Fact • Five day
Page 227 and 228: Prewitt Reservoir Explanation • P
Page 229 and 230: Prewitt Mystery Solved? • Campylo
Page 231 and 232: Future Prymnesium Problems in Wyomi
Page 233 and 234: Rapid Tests for the Detection of Pr
Page 235 and 236: Why rRNA probes? * universally foun
Page 237 and 238: PWD RP T3200-1203
Page 239 and 240: EUK 1209R CHLO 02 PRYM 02 PELA 01 P
Page 241: Change from PFA to ETOH Saline Chan
Page 245 and 246: PWD RP T3200-1203
Page 247 and 248: Harmful Algal Blooms Noctiluca Kare
Page 249 and 250: PWD RP T3200-1203 Coccolithus pelag
Page 251 and 252: Application & detection methods for
Page 253 and 254: Conclusions • Specific probes cou
Page 255 and 256: 1. ChemScanRDI, a laser based syste
Page 257 and 258: ChemScanRDI combines fluorescent ce
Page 259 and 260: Electrochemical detection of toxic
Page 261 and 262: Electrochemical Detection of rRNA f
Page 263 and 264: Comparison of cell counts with elec
Page 265 and 266: Long term stability of treated sens
Page 267 and 268: Development of DNA-microarrays for
Page 269 and 270: Scheme of a DNA-Chip Experiment Flu
Page 271 and 272: Monitoring Phytoplankton compositio
Page 273 and 274: EUK 1209 CHLO01 Localization of the
Page 275 and 276: Identification of Phytoplankton on
Page 277 and 278: DNA CHIP Clone librairy Dec 2000 1
Page 279 and 280: Research Needs • Monitoring of to
Page 281 and 282: Rapid Tests for the Detection of Ha
Page 283 and 284: abs. units 1.0 0.8 0.6 0.4 0.2 y =
Page 285 and 286: Prymnesium parvumRL10 % lysis 100 8
Page 287 and 288: PWD RP T3200-1203 Allelochemical ef
Page 289 and 290: Controlling Harmful Algal Blooms Do
Page 291 and 292: Management of harmful algae • Pre
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Chemical control of freshwater alga
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•This (small) reservoir had a lon
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Chemical flocculants - Phosphorus C
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PWD RP T3200-1203 from: River Scien
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Biological Control - Viruses Aureoc
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Viruses for HAB species Target spec
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Biological Control - Bacterial path
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Biological Control - Grazers Target
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Clay control of HAB species clay mi
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Variable removal ability of domesti
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PWD RP T3200-1203 Source: Sengco et
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Flume studies, WHOI PWD RP T3200-12
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Brevetoxin analysis cell concentrat
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Impacts - Benthic fauna Archambault
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Future directions: Cell removal, se
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When the cells reached exponential
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Conclusions Kalmar Experiments Phos
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Experiments at Woods Hole (in colla
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Recent work in Kalmar (data from J.
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Conclusions - control of Prymnesium
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A review of fish-killing microalgae
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Toxic/harmful microalgae • dinofl
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PWD RP T3200-1203 Fish kills
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Ichthyotoxic species • Karenia br
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FMRI,FWC Exposure routes • gills
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Gymnodinium pulchellum (brevetoxins
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Reef fish disease - Caribbean, Flor
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Diatoms • physical damage to gill
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Aquaculture in Israel and Prymnesiu
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Prymnesium parvum from Israel PWD R
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Prymnesium parvum blooms in Israel
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From Sarig 1971 Prymnesium parvum t
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From Sarig 1971 Mode of toxin actio
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Fish bioassay • dependence of tox
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Relationship # toxin concentration
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From Sarig 1971 Management of Prymn
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Management strategies • prevent b
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How to Use the Past to Plan for the
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How can studies on other harmful al
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Task Force report identified 7 HAB
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A 5-year federal, state, academic,
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ECONOMIC IMPACT In the 1970s, two r
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What is Needed Based on What is Kno
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Golden Alga Workshop Notes: Present
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facilities was a problem in North C
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Korean fisheries are a $1 billion i
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Friday October 24, 2003 Panel Discu
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• Design a sampling program with
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What are your recommendations for i
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• Rapid progress is made where th
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• Maintain a Golden Alga web site
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ChemScanRDI (a laser based system t
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• DNA-microarrays for monitoring
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• Reliable economic statistics fo
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• Be proactive; get messages out
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Golden Alga Workshop Attendees Firs
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Golden Alga Workshop Attendees, con
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Literature Review of the Microalga
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Oued Mellah Reservoir in Morocco oc
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the metal ions Fe, Zn, Mo, Cu or Co
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with the ability to utilize organic
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Figure 1. Prymnesin-1 (1) and Prymn
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Temperature Shilo and Ashner (1953)
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Glycerol Glycerol was found to incr
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since it seems that nitrogen and ph
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(Haptophyta) in semi-continuous cul
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Ulitzer, S. and M. Shilo. 1966. Mod
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