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ABI real-time PCR技术讲座(.pdf)

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TaqMan MGB 探 针<br />

R<br />

(non-fluorescent quencher) NFQ<br />

Q<br />

AGGCCTTGAGAGATAT<br />

Q<br />

MGB<br />

(minor groove binder)<br />

提 高 探 针 Tm 值<br />

R<br />

AGGCCTTGAGAGATAT Q<br />

| | | | | | | | | | | | | | | |<br />

GCTACACAGTCCGGAACTCTCTATAGCATCACAC<br />

R 报 告 荧 光 NFQ 无 荧 光 淬 灭 基 团 MGB 小 沟 结 合 物<br />

10

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