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ABI real-time PCR技术讲座(.pdf)

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两 种 荧 光 化 学 原 理<br />

●<br />

Fluorogenic 5’ Nuclease Assay<br />

– An established and proven chemistry for high specificity nucleic<br />

acid <strong>real</strong>-<strong>time</strong> quantitation<br />

– The introduction of TaqMan® MGB (Minor Groove Binder) probes<br />

has produced a turn-key homogeneous chemistry for the detection<br />

of Single Nucleotide Polymorphisms (SNPs)<br />

●<br />

SYBR® Green 1 Double Stranded DNA Binding Dye Assay<br />

– Ideal for target identification (screening) assays<br />

– A lower-cost alternative when high specificity is not required<br />

8

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