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ABI real-time PCR技术讲座(.pdf)

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斜 率 与 截 距<br />

●<br />

CT = - k lgX0 + b<br />

– Slope=-1/log(1+E)<br />

• If E=1, Slope= -3.3219280……<br />

• Normally, slope is about -3.5~-4.2<br />

– Intercept<br />

• Normally, intercept is about 30~40<br />

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