ABI real-time PCRææ¯è®²åº§(.pdf)
ABI real-time PCRææ¯è®²åº§(.pdf)
ABI real-time PCRææ¯è®²åº§(.pdf)
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高 分 辨 溶 解 曲 线 (HRM, High Resolution Melting )<br />
●<br />
Different from a regular SYBR ® Green dye melt curve:<br />
– Chemistry: Saturating and brighter dsDNA binding dyes<br />
• LCGreen ® EvaGreen TM SYTO ® 9<br />
– Instrument: More data points are collected<br />
– Software: New fluorescent normalization algorithms and<br />
plots<br />
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