ABI real-time PCRææ¯è®²åº§(.pdf)
ABI real-time PCRææ¯è®²åº§(.pdf)
ABI real-time PCRææ¯è®²åº§(.pdf)
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融 解 曲 线 (Dissociation curve)<br />
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Easily check for specificity of your SYBR assays<br />
immediately after PCR<br />
Thermal conditions (after last cycle of PCR):<br />
– Hold at 95°C for 20 sec<br />
– Hold at 60°C for 20 sec<br />
– Ramp and collect fluorescent data to 95°C over course of<br />
20 minutes<br />
Software automatically generates melt curve profiles<br />
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