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ABI real-time PCR技术讲座(.pdf)

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Calculating DNA Quantity of a Sample<br />

36<br />

34<br />

y = -1.2908Ln(x) + 27.187<br />

R 2 = 0.997<br />

32<br />

Sample<br />

30<br />

C T<br />

28<br />

26<br />

24<br />

22<br />

20<br />

0.001 0.010 0.100 1.000 10.000 100.000<br />

(Log N) initial concentration<br />

36

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