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ABI real-time PCR技术讲座(.pdf)

ABI real-time PCR技术讲座(.pdf)

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荧 光 参 比 - Passive Reference Dye: ROX<br />

ROX 以 固 定 的 浓 度 配 在 Master Mix 中 , 不 参 与 PCR 扩 增<br />

ROX 的 功 能 :<br />

Improves precision.<br />

Compensates for small fluorescent fluctuations that can occur from well-to-well<br />

Can be useful for diagnosing troubles<br />

Rn = Normalization = Reporter / Reference<br />

Reporter<br />

Reference<br />

20

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