ABI real-time PCRææ¯è®²åº§(.pdf)

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Allelic Discrimination Dye Components Viewer FAM VIC 46
阴阳性鉴定 Plus/Minus Assay 47
Page 1 and 2: 荧光 PCR 原理及应用周
Page 3 and 4: 美国 ABI 公司荧光定量
Page 5 and 6: ABI 公司荧光 PCR 仪器 App
Page 7 and 8: 荧光 PCR 的化学
Page 9 and 10: TaqMan 探针 5’ 3’ 5’ 3’
Page 11 and 12: MGB 探针的优点 ● ● ●
Page 13 and 14: SYBR Green 染料 SYBR Green I 与
Page 15 and 16: 融解曲线 (Dissociation curve
Page 17 and 18: Now can be performed on the ABI 750
Page 19 and 20: 荧光 PCR 的防污染技术
Page 21 and 22: ROX Normalizer 36 Replicates analyz
Page 23 and 24: 实时定量与终点定量
Page 25 and 26: 什么是 CT 值 ? 荧光信
Page 27 and 28: 斜率与截距 ● CT = - k lg
Page 29 and 30: 成功的定量 PCR 数据 29
Page 31 and 32: 荧光 PCR 技术的应用
Page 33 and 34: 定量 PCR - Quantitative PCR ●
Page 35 and 36: Results from RNase P Instrument Ver
Page 37 and 38: Data Report Table 37
Page 39 and 40: 相对定量 ● Relative gene e
Page 41 and 42: Relative Expression 41
Page 43 and 44: 等位基因鉴定机理野
Page 45: Allelic Discrimination Assay FAM VI
Page 49 and 50: Target vs. IPC Competition Target I
Page 51 and 52: 需要了解的问题 ● ●
Page 53 and 54: 7500 System Hardware Features - Opt
Page 55 and 56: 定量 PCR 的实验要素
Page 57 and 58: 样品 ● ● DNA 纯度 :OD260/
Page 59 and 60: 标准品梯度稀释方法
Page 61 and 62: PCR 效率对定量结果的
Page 63 and 64: ??? ● 在使用 TaqMan MGB 探
Page 65 and 66: 复管测试 ● ● 样品和
Page 67 and 68: Q & A 67
Page 69: 谢谢 ! 8008203939 cnmcbsupport@a

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