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ABI real-time PCR技术讲座(.pdf)

ABI real-time PCR技术讲座(.pdf)

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相 对 定 量<br />

●<br />

ΔΔCt<br />

– 相 对 量 = 2 -ΔΔCT<br />

– 目 标 序 列 和 内 参 序 列 的 扩 增 效 率 相 等 ( 且 接 近 100%)<br />

●<br />

The Relative Standard Curve Method<br />

40

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