User Guide: NanoLC Ultra® System Integration Test - Eksigent
User Guide: NanoLC Ultra® System Integration Test - Eksigent
User Guide: NanoLC Ultra® System Integration Test - Eksigent
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LC/MS <strong>System</strong> Configuration <strong>Test</strong>— AB SCIEX TripleTOF 5600 <strong>System</strong><br />
Note: For a trap-elute configuration, the Sample Injection method should<br />
be Standard.<br />
Figure 1-4<br />
6. Click Save.<br />
LC Method Settings dialog—Run Conditions tab<br />
Create the Gradient Method in the <strong>Eksigent</strong> Control Software<br />
For the analytical gradient (typically, the Gradient 2 pump with the nanoflow module), create the<br />
gradient method.<br />
1. Make sure that Gradient 2 is selected as the channel (top, right corner of window).<br />
2. Click LC Methods.<br />
3. If the installation includes a Nanoflex system, then in the Name field, type CH2<br />
15min 400nLmin nanoflex trap, and click Save.<br />
Or<br />
If the installation includes an external column, then in the Name field, type CH2<br />
15min 400nLmin trap, and click Save.<br />
4. On the Gradient Table tab, specify the method as shown in Figure 1-5 or Figure 1-7,<br />
depending on the installation.<br />
<strong>NanoLC</strong> Ultra ® <strong>System</strong><br />
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<strong>System</strong> <strong>Integration</strong> <strong>Test</strong><br />
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