User Guide: NanoLC Ultra® System Integration Test - Eksigent

LC/MS System Configuration Test— AB SCIEX TripleTOF 5600 System
8. Make sure the peaks have good separation and shape using the PeakView software.
i. In the PeakView software, on the Window menu, click Graph Selection
Window.
ii. Note the retention times of the chosen peaks.
This will vary with each system. Time of elution of the first is about 14 to 16
minutes. The longer elution time indicates you need to minimize LC dead
volume.
Note: Most of the XICs obtained should have peak widths of ~0.17 minute
half height on average and have peak intensities similar to that shown in
Figure 1-29. Some peaks will be narrower and some will be broader.
Measure the Performance of the TOF MS/MS Scan
1. In the main TIC pane, extract individual TICs by double-clicking the magenta arrow.
2. Select the MS/MS scan, and then select Remove all Traces Except Active to
display the pane shown in Figure 1-30.
Figure 1-30 TOF MS/MS XIC of m/z 729.3652 peak
NanoLC Ultra ® System
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System Integration Test
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