User Guide: NanoLC Ultra® System Integration Test - Eksigent
User Guide: NanoLC Ultra® System Integration Test - Eksigent
User Guide: NanoLC Ultra® System Integration Test - Eksigent
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LC/MS <strong>System</strong> Configuration <strong>Test</strong>— AB SCIEX TripleTOF 5600 <strong>System</strong><br />
Verify <strong>System</strong> Readiness<br />
Make sure the <strong>NanoLC</strong> Ultra system is meeting performance specifications.<br />
1. Connect effluent from the <strong>NanoLC</strong> Ultra system to the NanoSpray ion source and<br />
verify that the spray is stable by monitoring the background signal in the Analyst TF<br />
software.<br />
2. Equilibrate the LC/MS system with the starting conditions of the method outlined<br />
above.<br />
3. Make sure the spray is stable.<br />
4. Double-click Manual Tune in the left Navigation bar.<br />
5. Enter the key parameters in Table 1-3, and then click Start to begin acquisition.<br />
Table 1-3 Key Parameters<br />
Parameter<br />
Value<br />
MS<br />
Scan type<br />
TOF MS<br />
Polarity<br />
Positive<br />
Start Mass 400<br />
Stop Mass 1000<br />
Run Time<br />
2 min<br />
Source/Gas**<br />
Curtain Gas (CUR) 20-25<br />
IonSpray Voltage (IS) 2100-2400 V<br />
Ion Source Gas 1 (GS1) 2-15<br />
Interface Heater 150°C<br />
Compound<br />
Declustering Potential (DP) 70<br />
Figure 1-22 Analyst TOF MS scan for background noise<br />
<strong>NanoLC</strong> Ultra ® <strong>System</strong><br />
36 of 124<br />
<strong>System</strong> <strong>Integration</strong> <strong>Test</strong><br />
D5030175 A
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