User Guide: NanoLC UltraÂ® System Integration Test - Eksigent

More documents

Recommendations

Info

Contents Condition the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .74 Verify System Readiness . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .74 Perform the System Functional Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76 Perform the System Performance Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78 Troubleshoot Peak Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Create a Backup of the EKSettings.reg File . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Test Results—QTRAP 5500 Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 Chapter 3 LC/MS System Configuration Test— AB SCIEX 4000 QTRAP ® System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Create the Methods and Batch for the System Functional Test . . . . . . . . . . . . .86 Create the Acquisition Method and Batch—Functional Test . . . . . . . . . . . . . .86 Create the LC Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88 Create the Acquisition Method in the Analyst® Software . . . . . . . . . . . . . . . .93 Create the Methods and Batch for the System Performance Test . . . . . . . . . . .97 Create the Acquisition Methods and Batch—Performance Tests . . . . . . . . . .97 Create the LC Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .98 Create the Acquisition Method in the Analyst Software . . . . . . . . . . . . . . . . .103 Prepare the System for Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 Prepare the Solution and Dilution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 Condition the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 Verify System Readiness . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108 Perform the System Functional Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .110 Perform the System Performance Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .112 Troubleshoot Peak Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .114 Create a Backup of the EKSettings.reg File . . . . . . . . . . . . . . . . . . . . . . . . . . .116 Test Results—4000 QTRAP Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . .117 Appendix A System Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 Prepare the [Glu 1 ]-Fibrinopeptide B Dilution . . . . . . . . . . . . . . . . . . . . . . . . . . .119 Edit the Calibration Reference Table for [Glu1]-Fibrinopeptide B . . . . . . . . . . .120 Calibrate the TOF MS Scan Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121 Calibrate the TOF MS/MS for High Sensitivity and High Resolution Product Ion Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 NanoLC Ultra ® System 4 of 124 System Integration Test D5030175 A
Foreword This foreword contains general safety-related information, describes the symbols and conventions used in the documentation, and provides regulatory compliance information. It also describes potential hazards and associated warnings for the system, and the precautions that should be taken to minimize the hazards. In addition to this foreword, refer to the Site Planning Guide for site requirements. General Safety Information Before operating any instrument, become familiar with its operation and with the potential hazards. To prevent personal injury or instrument damage, read, understand, and obey all safety precautions. Warnings in this document and labels on the device are shown with international symbols. Failure to heed these warnings could result in serious injury. This safety information is intended to supplement federal, state or provincial, and local environmental health and safety (EHS) regulations. The information provided covers instrumentrelated safety with regard to the operation of the device. It does not cover every safety procedure that should be practised. Ultimately, you and your organization are responsible for compliance with federal, state or provincial, and local EHS regulations and for maintaining a safe laboratory environment. For more information, refer to the appropriate laboratory reference material and standard operating procedures. Symbols and Conventions The following conventions may be used throughout the guide. DANGER! Danger signifies an action which leads to severe injury or death. WARNING! Personal Injury Hazard: A warning indicates an operation that could cause personal injury if precautions are not followed. WARNING! Electric Shock Hazard: This symbol indicates a warning of electrical shock hazard. Read the warning and follow all precautions before performing any operation described in the guide. Failure to do so can result in serious injury. WARNING! Burn Hazard: This symbol indicates a warning of potential burns from hot surfaces. Read the warning and follow all precautions before performing any operation described in the guide. Failure to do so can result in serious injury. System Integration Test D5030175 A NanoLC Ultra ® System 5 of 124
Page 1 and 2: NanoLC Ultra ® System for TripleTO
Page 3: Contents Foreword. . . . . . . . .
Page 7 and 8: Foreword WARNING! Potential System
Page 9 and 10: Foreword Mains Supply WARNING! Elec
Page 11 and 12: Foreword Additional Documentation
Page 13 and 14: LC/MS System Configuration Test—
Page 55 and 56:
LC/MS System Configuration Test—
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Prepare the System for Testing LC/M
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Prepare the System for Testing LC/M
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
System Calibration A If the NanoLC
Page 121 and 122:
System Calibration Calibrate the TO
Page 123 and 124:
System Calibration Table A-2 TOF MS
show all

User Guide: NanoLC UltraÂ® System Integration Test - Eksigent

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?