User Guide: NanoLC Ultra® System Integration Test - Eksigent
User Guide: NanoLC Ultra® System Integration Test - Eksigent
User Guide: NanoLC Ultra® System Integration Test - Eksigent
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LC/MS <strong>System</strong> Configuration <strong>Test</strong>— AB SCIEX TripleTOF 5600 <strong>System</strong><br />
Figure 1-26 TOF MS XICs of 5 peptides from Beta-Galactosidase <strong>NanoLC</strong> Ultra trap elute<br />
run (200 fmol on column)<br />
Note: This test is not a specification. Use this example to confirm injection<br />
and peak separation and shape only.<br />
6. Make sure the peaks have good separation and shape.<br />
i. In the PeakView software, on the Window menu, click Graph Selection<br />
Window.<br />
ii. To collect the Peak Area for each XIC, click each (color-keyed) line in the<br />
upper left.<br />
The retention time appears on the top of the peak selected. The bottom line of<br />
the Graph Selection Info displays the Peak Area.<br />
Measure the Performance of the TOF MS/MS Scan<br />
1. In the main TIC pane, extract individual TICs by double-clicking the magenta arrow.<br />
2. Select the MS/MS scan, and then select Remove all Traces Except Active to<br />
display the pane shown at the bottom of Figure 1-27.<br />
<strong>NanoLC</strong> Ultra ® <strong>System</strong><br />
40 of 124<br />
<strong>System</strong> <strong>Integration</strong> <strong>Test</strong><br />
D5030175 A
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