User Guide: NanoLC Ultra® System Integration Test - Eksigent
User Guide: NanoLC Ultra® System Integration Test - Eksigent
User Guide: NanoLC Ultra® System Integration Test - Eksigent
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LC/MS <strong>System</strong> Configuration <strong>Test</strong>— AB SCIEX TripleTOF 5600 <strong>System</strong><br />
<strong>Test</strong> Results—TripleTOF 5600 Instruments<br />
For TripleTOF 5600 instruments, complete this table with the results from the ion from the Beta-<br />
Galactosidase digest solution stock. Make sure that the peak area is within specification.<br />
Most of the XICs obtained should have peak widths of ~0.17 minute half height on average and<br />
have peak intensities similar to that shown in Figure 1-29. Some peaks will be narrower and<br />
some will be broader Additionally, verify that the peaks elute within 5 minutes of each other.<br />
Beta-Gal Lot Number: ___________________________________<br />
Table 1-4 LC/MS Specification <strong>Test</strong>—TripleTOF 5600 Instruments<br />
Q1 Peptide ID Spec.<br />
(peak area)<br />
503.2368 YSQQQLMETSHR 1.0E+05<br />
542.2645 GDFQFNISR 1.0E+05<br />
671.3379 VDEDQPFPAVPK 2.0E+05<br />
714.8469 DWENPGVTQLNR 5.0E+04<br />
729.3652 APLDNDIGVSEATR 2.0E+05<br />
Specification Passed<br />
Notes<br />
Actual<br />
<br />
<strong>NanoLC</strong> Ultra ® <strong>System</strong><br />
50 of 124<br />
<strong>System</strong> <strong>Integration</strong> <strong>Test</strong><br />
D5030175 A
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