User Guide: NanoLC Ultra® System Integration Test - Eksigent

LC/MS System Configuration Test— AB SCIEX TripleTOF 5600 System
Figure 1-24 Add Samples dialog—System Functional Test
3. Click OK.
4. Save the data file as TT5600 system LC BGal functional status check .
5. On the Location tab, specify the location of the Beta-Galactosidase sample in the
autosampler.
Run the Batch
1. On the Submit tab, click Submit.
2. In the View menu, click Sample Queue.
3. In the Acquire menu, click Start Sample.
Verify the Results in the PeakView Software
1. Open the data file and double-click the magenta arrow to display the individual TICs.
2. Right-click the TIC of the TOF MS and select Remove all Traces Except Active to
display the overlaid TOF MS TIC.
3. Extract the TOF MS peak XICs for the target peptides.
i. On the Show menu, click Extract Ions Using.
ii. Specify the masses and extraction width for the peptides as shown in
Figure 1-25. Only the masses and widths are required.
NanoLC Ultra ® System
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System Integration Test
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