Real time PCR - European Pharmaceutical Review

qPCR ISSUE 2009
Figure 11: Chromatin immuno-precipitation (ChIP). In this technique, intact cells are fixed using formaldehyde, which cross-links and
preserves protein/DNA interactions. The DNA is then sheared into small, uniform fragments using sonication and specific
protein/DNA complexes are immunoprecipitated using an antibody directed against the DNA-binding protein of interest.
Following immunoprecipitation, cross-linking is reversed, the proteins are removed by treatment with Proteinase K and the DNA is
purified. The DNA is then analysed to determine which DNA fragments were bound by the protein of interest.
(Adapted from Chromatin Immunoprecipitation Kits, Active Motif North America)
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Dr. Frédérique Ponchel
Dr. Frédérique Ponchel currently works as
a RCUK Senior Academic Research Fellow
at the Leeds Institute of Molecular
Medicine at Leeds University. Prior to this
Frédérique has worked at The University of
York as a postdoctoral fellow. Originally
from France, Frédérique studied
Biochemistry and Genetics in Paris.
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