impact of latex and plant extract of and the recovery of latex toxicity ...

in the normal survival of organisms over a prolonged period of time. Accordingly,
the present study was taken up with sub lethal dose response in the experimental fish.
The 96 hours LCso was considered as One Toxic Unit (ITU) (Sprague and Ramsay,
1965) and based on this value, three different sub lethal concentrations viz.,
1/~'~,1/10" and 1120'~ of LC50 values (TUl(0.005 mU5litre); TU2(0.01 mlI5lihe) and
TU3 (0.02 ml/Slihe) of
latex, for plant extract TUl(0.5 mUSlitre);TU2(1
ml/Slitre);TU3(2 mU5litre) and three different sub lethal concentrations viz.,
115'~,1/10" and 1120~ of LC50 values (TUl(O.(X)S mV5lihe); TU2(0.01 mU5litre) and
TU3 (0.02 mV5lihe) of latex plus supplements was taken for biochemical,
behavioural, haematological, histopathological studies. Behavioural study,
biochemical, enzyme assay and haematological studies were performed for 96hrs.
Histopathological studies were also carried out for 96hrs of exposure at three
sublethal concentrations as mentioned above. A detailed description of the
methodology for acute toxicity test and the sublethal tests is described in the
respective chapters.
Statistical Analysis:
The data are presented as the Mean * SEM (Standard Error of Mean).Variations
among the sample means is due to sampling errors. Standard Error is an important
instrument which measures sampling variability due to chance or random forces, with
this we determine limits with in which the parameter values are expected to lie. Here
significant treatment effect was found, the students "t" test was used to compare the
experimental groups with their respective controls, for each exposure period (Ode
,1966).From the degree of freedom, values of probability were obtained from the
standard table given by Fisher and Yates (1948). If the calculated value is more
than the table value, it is significant at the probability level. The following levels of
significance were used p