impact of latex and plant extract of and the recovery of latex toxicity ...

changes in protein as a function of the extent of contamination on the nature of the
pesticide. However some show alterations is the mobility of protein patterns in
response to pesticides (Reeta Pundey et uL, 1991).The impact of the latex, latex with
supplements and plant extract of C.g~gantea(L.)R.Br. was thus taken for present
to find if any alteration in protein content had taken place, because depletion of
the protein content could be due to its util~zation as an energy source, thus ind~cating
that other energy source had already been used up to a formidable extent and the
organisms is on the brink of death; cell damage, thus influencing protein synthesis
and causing disruption of many physiological and metabolic processes.
hlaterials and Methods:
The details of collection, maintenance and acclimation of the fish Anabas
resludineus, the experimental procedures followed In the present study are detailed in
the earlier chapters. The fishes were exposed to TUI (0.005m1/51itre);
TU2(.0lml/5litre) and TU3 (.02mll5litre) of 96hr LC50 concentration of latex, latex
w~th supplements and for plant extract TU1(0.5ml/5litre); TU2(lml/5litre);
TU3(2mlISlitre) of 96 hr LC50 was taken. The Proteln concentrations in gill, liver
and brain are determined by analyzing the tissues of 96hrs exposure.
Preparation of Tissue Homogenate:
The fishes after subject to sacrifice, the organs were dissected. The tissue was
weighed for 100 mg, minced and cleaned in saline and homogenized in 2 ml of Tris-
HCL buffer @H 7.5, 0.1 M) using a homogenizer at 4'~.~he homogenate was
centrifuged at 12,000 rpm, for 30 minutes. The supernatant was carefully removed
Into another tube and used as tissue extract.
Estimation of protein:
The estimation of protein concentration was done by the method of Lowry et uL,
(1951) using crystalline bovine serum albumin (BSA) as the standard.