impact of latex and plant extract of and the recovery of latex toxicity ...

paper. To the precipitate 1 ml of distilled water was added and re-dissolved in 1.25
ml of 95% ethanol. The Supernatant was decanted off and the tube was drained on
filter paper as before. The precipitate was then dissolved in 5 ml of distilled water
and placed in ice bath to this 10 ml of anthrone reagent was added and boiled in a hot
water bath. Glass marbles were kept on the tubes while boiling to avoid the overflow
of reagents. A green colour was developed which was then red in a
spectropbotometer at 620nm after cooling the tube. Similar procedure was employed
for preparing "blank" and standard solutions.5 ml of water and 5 ml of standard
solution containing 100 pg 1 I of glucose treated with 10 ml of anthrone reagent
sewed as blank and standard respectively.
The glycogen content in aliquot was calculated by using the foilowing
equation:
Glycogen in aliquot (mg ) I00 X U
1.11 X5
Where, U - Optical density of unknown solution
S - Optical density of 10 pg 11 glucose standard
1.11 - the factor determined by Moms, 1948.
For the convesion of glucose to glycogen with this equation. The glycogen content
of the tissue was expressed as mg / gram tissue.
Toxicity Impact on Acid and Alkaline Phosphatase Activity
Acid Phosphatase (E.C 3.1.3.2) and Alkaline Phosphatase (E.C. 3.1.3.1) are group of
widely distributed enzymes of very broad specificity. They act on a wide range of
nonoesten of orthophosphoric acid, both aliphatic and aromatic (Fernley and
Walker, 1971). They are known as "inducible" enzymes whose activity in animal
tissue goes up when there is a toxic impact and the enzyme begins to counter act.
Subsequently the enzyme's activity may begin to drop either as result of having