impact of latex and plant extract of and the recovery of latex toxicity ...

Procedure:
To the tubes labeled 'test' and 'blank' 0.5 ml of para-nitrophenyl phosphate and 0.5
ml of glycine buffer was added. The tubes were placed in a water bath at 37'~
for 5
minutes. The reaction was initiated by the addition of 0.1 ml of sample to the test
and 0. I ml of distilled water to the blank. Exactly after 30 minutes of incubation at
37'~.~he reaction was arrested by addition of 10 ml of NaOH. The tubes were mixed
well and the colour developed was read at 410 nm, in a spectrophotometer, against
the blank. 0.1 ml of concentrated Hydrochloric acid was added, mixed and the optical
density was read at 410 nm against the blank. The difference in the two absorbance
was taken as the measure of enzyme activity. Suitable standards were mn along with
the assay. The alkaline Phosphatase activity was calculated from the calibration
curve obtained using para-nitrophenol standard. The enzyme activity is expressed as
p moles of para nitrophenol formed per hour per mg protein.
Toxicity Impact on Acetylcholinesterase
The important toxic property of pesticides is inhibiting their target enzyme acetyl
cholinesterase (AChE), and as well studied (O'Brein, lP67;Corbelt,1974),most of
the chemical pesticides are similar with the ester part of the acetylcholine and they
react with esterase part of AChE after entering into the exposed fish The conversion
of acetic acid and choline catalysed by AChE is considered to be the reaction in
synaptic transmission(Bachelard,l976).Decrease in AChE activity and increased
accumulation of AChE was observed in fresh water mussel treated with
demacron(Vijendrababu and Vasudev,l984),with Fenotrothion in Senex senex
(Bagyalarmi and Ramamur~,l980) and many organophosphates in fishes were
reported(March et a1 .,1956;Murphy el aL,1968).A drop in AChE in vertebrates
caused various neurobiological changes that reduce the animal survival ability.
Fishes were exposed to three sublethal concentrations of latex, latex with
supplements and plant extract. The tissues of gill, liver and brain excised and are
used for the following experiment.