Protein biosynthesis: aminoacyl-tRNA synthetases and aminoacylation

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Aminoacylation reaction • there is a different AaRS for each amino acid (20 in all) in most cells • recognition of amino acid and tRNA varies among AaRSs • all AaRSs catalyze the same 2-step ATPdependent reaction (1) aaRS + ATP:Mg2+ + aa ↔ aaRS:aa:AMP + PPi :Mg2+ (2) aaRS:aa:AMP + tRNA ↔ aaRS + aa-tRNA + AMP
Page 1: Protein biosynthesis: aminoacyl-tRN
Page 4 and 5: Comparison of class I and class II
Page 6 and 7: Evolution and sub-groups of AaRS
Page 8 and 9: Class II active site, 3 conserved m
Page 10 and 11: Recognition of tRNAs by AspRS (clas
Page 13 and 14: The structure of the complex betwee
Page 16 and 17: LysRS of most eukaryotes and bacter
Page 19 and 20: • inhibiors of AaRSs can be used
Page 21: tRNA
Page 24: Sequence elements: Mostly non-modif
Page 27 and 28: Structural elements: identity eleme
Page 29 and 30: • Anti-determinants: negative sig
Page 31: • identity elements mostly cluste
Page 36 and 37: The result of aminoacylation by a c
Page 38 and 39: • some class I AaRS (GlnRS, GluRS
Page 40 and 41: Active site structure of ThrRS, rec
Page 42: AaRSs use chemical properties, size
Page 45 and 46: Absolute accuracy of aminoacylation
Page 47 and 48: • some AaRS (IleRS, ValRS, LeuRS
Page 49: Pyrococcus horikoshii LeuRS-tRNALeu
Page 52 and 53: Unusual aminoacylation mechanisms E
Page 54 and 55: RS D-GluRS: C36 - Arg358 discrimina
Page 57: 4. Some thermophilic archaea (Metha
Page 62 and 63: •aminoacylation of tRNASec by Ser
Page 64: MTF

Protein biosynthesis: aminoacyl-tRNA synthetases and aminoacylation

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