Protein biosynthesis: aminoacyl-tRNA synthetases and aminoacylation
Protein biosynthesis: aminoacyl-tRNA synthetases and aminoacylation
Protein biosynthesis: aminoacyl-tRNA synthetases and aminoacylation
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• some AaRS (IleRS, ValRS, LeuRS <strong>and</strong> ThrRS)<br />
have editing sites in addition to acylation sites<br />
• these two sites function as a a double sieve to<br />
ensure a very high fidelity<br />
• wrong amino acid cannot bind to active site <strong>and</strong><br />
correct amino acid is rejected by the editing site<br />
• hydrolysis of correct product is slow <strong>and</strong> hydrolysis<br />
of the “wrong” product is quick<br />
• in case of IleRS 80% of editing is pre-transfer, in<br />
case of ValRS <strong>and</strong> LeuRS mostly post-transfer<br />
editing (double sieve)
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