Biennial Report 2005-2007 - Saha Institute of Nuclear Physics

206 Biennial Report 2005-07Fig.6.1.2.1. Ribbon model of LdCyp showing active site residuesL.donovani adenosine kinase (LdAdK) and Arg147 in LdCyp was found to play a key role inreactivating LdAdk. Cyclophilin mutant (R147A) has been crystallized and also the molecularbiology of calcineurin and some other proteins has been initiated.V Venugopal, Banibrata Sen†, Alok K Datta†, Rahul BanerjeeC&MB6.1.2.2 Sequencing, cloning and expression of the most thermostable Ervatamin,Ervatamin-COur previous studies on Ervatamins established that Ervatamin-C shows unusual stability amongErvatamins and other related proteases. On the basis of this observation we have initiated this programprimarily to understand the role of individual amino acid residues to maintain the structuralintegrity of the thermostable protein at the molecular level. We have sequenced the Ervatamin-CcDNA and cloned the cDNA in the expression vector.Raka Ghosh, JK Dattagupta, Sampa BiswasC&MB6.1.2.3 Structure-function correlation of Cysteine proteases from the latex of a medicinalplant Ervatamia coronariaErvatamin-A, -B and -C, three papain-like cysteine proteases have been isolated from the latex ofthe plant, characterized and X-ray structures of the three have been solved in the previous years.The thermal stability of the individual proteases have been determined from temperature inducedCD spectra and compared among themselves and with other members of the family. The structural