Operation Manual - Mrclab.com

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CHAPTER THREE UV DetectorHPLC-8100 HPLC ChromatographBrief descriptions of UV Detector1. General descriptionBFRL UV detector is a valuable new type detector,especially developed for the routine analysis.The deuterium lamp is used as the light source of the instrument.UV detector selects the flow cell of standard type(Optical path:10mm,volume:10µL,the flowrate range of eluent phase of UV detector is available from 0.001~9.999mL/min.After powering up, the UV detector performs the self test and calibration automatically.● The internal software includes an auto-calibration of the wavelength scale. Theposition of the grating reflection (0. order) will be located automatically.● The deuterium lamp with the wavelength of 656nm is used to check and to calibratethe display.The distance of the wavelength passed through the monochromator should be 1nm for perstep, arbitrary setting can be made in the range of 190nm to 740nm. Accuracy is ±2nm.In addition, UV detector still have the function of the whole wavelength or the wavelengthrange set by user to process stopping the flow scanning.UV detector provides with very low noise(2×10 -5 AU)and shift(15×10 -5 AU/h),availablefor auto-zeroing of the full scale.Key pad is clear, clean film, convenient for <strong>com</strong>pleting the operation.The control and data acquisition of the UV detector is digitalization to secure runningwithout the trouble.2. Optical path of the UV detector1. Deuterium lamp2. Slit3. Filter4. Concave mirror5. Plane grating monochromator6. Half transparent mirror7. Flow cell8. Photodiode (Sample)9. Photodiode (Reference)Fig. 6Optical Path of UV detectorInstructions for optical pathA beam of emitted light from the deuterium lamp(1)is faded out by a slit(2). After passing afilter(3) it is focused by a concave mirror(4)on a plane grating monochromator(5),soas to obtain a beam light of characteristic wavelength. The bream is then split by a halftransparent mirror(6). One part beam delivers the reference signal(9),and the other afterpathing the flow cell(7)irradiates to photodiode (8) to produce the measurement signal.11
HPLC-8100 HPLC ChromatographPreparation of UV detector before the operation:1. Front view of UV detector1.Display information area 2.Foil key pad 3.UV detcector front doorFig.7Display area and foil key padFront panel of UV detector and flow cellInformation display areaFig. 8 Display area and foil key pad1. Display area 2. Control function key3.Input key4. Arrow key and ENTER keyABS-0.0001 AUWAVELAMP IS LIT254 nmFig. 9Display information areaInformation display area displays real time parameters and result data of UV detector.Foil key pad areaFoil key pad includes control function key(Auto-zeroing key and running program key),Data12
Page 2 and 3: HPLC-8100 HPLC ChromatographCONTENT
Page 4 and 5: CHAPTER ONE PREFACEHPLC-8100 HPLC C
Page 6 and 7: Connections of piping (3 and 4 in F
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Page 13: HPLC pump technical parametersFlow
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Page 19 and 20: HPLC-8100 HPLC ChromatographD2 LAMP
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Page 23: Technical data of UV detectorWavele
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Operation Manual - Mrclab.com

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