Operation Manual - Mrclab.com

HPLC-8100 HPLC ChromatographSimple Maintenance1. Lamp maintenanceThe deuterium used with UV detector has an extended lifetime to ensure long-time functionalityand reliable measurements with low noise and baseline drift as well as high sensitivity. (life timeabout 2000 hours). The actual using time of the deuterium lamp depends on different factors, likethe number of lamp starts, the average burning time and your requirements concerning noise andsensitivity.To check the functionality of the lamp, the two intensity values of sample and reference (see“D 2lamp energy and used time” menu),the help information for D 2lamp can be found there. Thereference value refers to the intensity of the light measured in the reference channel and be usedfor checking the quality of the lamp.We recommend to check the reference value at regular intervals under the conditions mentionedabove(dummy cell, λ= 240nm). This applies especially in case higher noise levels or decreasedsensitivity is observed on working with UV detector. In these observations coincide with areference value of approx. 0.1 or less, or used time is over 2000h, a new deuterium lamp shouldbe installed.2. Cleaning the flow cellNoisy baselines and low sensitivities may be due to a dirty flow cell. This may also be indicatedby a low absorbance value in the signal menu when flushing the cell with pure solvent. Thatshows the flow cell has been polluted. In most cases it is sufficient to purge the flow cellaccording to the following SOP.SOP 1 Purging the flow cellThis instruction applies to the flow cell delivered with UV detectora) Purge the flow cell using one of following solvents: sodium dodecyle sulfate(SDS), 1m HCL, 1m NaOH, ethanol, or acetone.b) Run the solvent through the flow cell using a syring and leave for approximately 5minutes.c) Rinse extensively with water and the blow dry using a gentle stream of purenitrogen.CAUTION: Never dry with compressed air to blow the flow cell, this will containmicrodroplets of oil that will coat the cell.CAUTION: When the optics module is not in use, disconnect the flow cell and cleanouter traces of salt and protein with a syringe filled with distilled water.Before storing the flow cell inject a dilute solution (10-25%) of ethanol ori-propanol to prevent microbial growth.CAUTION: In case the flow cell purging do not provide sufficient success, all flow cellscan easily be disassembled for cleaning the lenses.18