Operation Manual - Mrclab.com

HPLC-8100 HPLC ChromatographSetting of detectorFig. 5The pump requires to set the control mode (control mode divides: single pump, double pumprunning independently, double pump high pressure gradient and single pump low pressuregradient), If using gradient table, pump flow and up limit and down limit of pressure; Detectorrequires to set the wavelength and deuterium lamp status, if using the wavelength program table,etc. The sensitivity of the detector is set by the acqueuiescence. The spectral scanning is setaccording to the concerning conditions when it is required.2. Operation procedures of work stationUse the work station software to do the experiment after the settings mentioned above. Asexample for outer standard method as follows:Operation procedures of outer standard method1. Create a new method file parameter to do the experiment. Its procedures are as follows:1) Start HPLC chromatograph and work station software;Fig. 627