Conference Program - ABRF 2011 - Association of Biomolecular ...

Vendor PresentationsSunday, February 20 — 12:00 pm – 1:30 pmAlyssum Room, Level 3Exhibit Booth: 214Moving Beyond ProteomicsSpeaker: Detlev Suckau, Bruker Daltonik GmbHIn current proteomics studies as well as biopharmaceutical developmentand characterization, post-translational modificationsobtain increasing attention. Amongst these, glycosylation isprominent but the lack of bioinformatics solutions slowed downthe analysis of glycoproteins in both, the biopharmaceutical industryas well as in proteomics studies.In this seminar, we introduce new solutions that include a newglycoprotein and glycoproteomics enabling bioinformatics platformand show examples from selected glycoprotein characterizationsincluding glycan and direct glycopeptide analysis usingBrukers leading ESI-MSn and MALDI platforms.Dedicated solutions for the development and quality control ofBiologicals and Biosimilars will be described that show you howto rapidly characterize therapeutic proteins with the confidencethat is derived from the high specificity of Brukers ultra-highresolution QTOF maXis.As a further extension of classical protein characterization andvalidation workflows, we describe recent advances in Top-Downprotein characterization tools that will make your C- and N-terminaldefinitions specific and rapid. The ESI-ETD and MALDITop-Down Sequencing approaches will also forward your proteomicsresearch as they provide simultaneous access to PTManalysis, protein isoform differentiation and proteolytic regulationevents that are not addressed with current technologies.Grand Oaks Ballroom,Rooms N&O, Level 2Exhibit Booth: 71412:00 pm – 12:30 pmAdvances in Sample Preparation of LowAbundant Proteins using Magnetic BeadsSpeaker: Helena Hedlund, Global Product Manager, GEHealthcare Life SciencesLow abundant proteins are often biologically important butdifficult to detect. Some proteins are present in a few copieswhereas others have a short transient presence. To be able toincrease the detection and identification rate, efficient samplepreparation methods are necessary. We have combined establishedaffinity chromatography methods with magnetic beadtechnology for the enrichment of such challenging proteins. Inthe first study changes in tyrosine phoshorylation in cancer cellsupon drug treatment was investigated. Immmonoprecipitationusing Protein G Mag Sepharose TM was used for enrichment ofphosphorylated proteins prior to a 2-D DIGE experiment. Theeffect of the treatment will be presented as well as protein identificationfrom MS analysis.In the second study screening for optimal purification conditionsfor a number of integral membrane proteins was performed. Vitalparameters as choice of detergent wash and elution bufferconcentrations were evaluated. All experiments were done usingNi2+-charged His Mag Sepharose Ni. Results from screeningfor several detergents gave reliable results that could be fed intofurther scale-up experiments. The magnetic bead format simplifiedand shortened the handling procedures through-out thesetwo different workflows.Vendor Presentations12:30 pm – 1:00 pmQuantitative Western Blotting withAmersham TM ECL TM PrimeSpeaker: Maria Winkvist, Scientist, GE Healthcare LifeSciencesWestern blotting is a well established technique in life scienceresearch. Traditionally the technique has been restricted toqualitative protein analysis. However, development of refineddetection methods and reagents has opened up the possibilityto use the technique in quantitative analysis, for accurate monitoringof small changes in protein abundance and for detectionof posttranslational modifications.120 • ABRF 2011 — Technologies to Enable Personalized Medicine