Conference Program - ABRF 2011 - Association of Biomolecular ...

www.454.comSequence-Enrichment SolutionGS Junior andSequence CapturePerform targeted resequencing of any genomic region in your own labby combining two new Roche technologies: NimbleGen EZ ChoiceSequence Capture custom probes and the GS Junior Instrument.Use an easy, scalable workflow for sequence enrichment —Choose a simple workflow featuring gel-free sequencing librarypreparation, single-tube capture with stable DNA probes, and easyhybridization setup.Reduce sequencing costs — Multiplex sequencing samples withmolecular barcodes to maximize data output per run.To learn more, visit www.454.com Figure 1: The GS Junior Sequence Capture Protocol.1. The genomic DNA sample is fragmented and polished.2. Linkers are ligated to the fragments.3. The sample is hybridized to a library of DNA capture probes.4. Unbound fragments are washed away.5. The captured genomic DNA (bound to beads) is used directly as PCR template.6. The amplified, enriched sample is ready for high-throughput sequencing withthe GS Junior Instrument.For life science research only.Not for use in diagnostic procedures.454, 454 SEQUENCING, NIMBLEGEN, and GS JUNIOR are trademarks of Roche.© 2011 Roche Diagnostics. All rights reserved.Roche Diagnostics CorporationRoche Applied ScienceIndianapolis, Indiana