Conference Program - ABRF 2011 - Association of Biomolecular ...

Phoenix crystallography robot and a LC-MALDI-TOF/TOF (an ABI 4700with a LC Packings Ultimate Nano-LC system with a Probot spottingrobot). In the College of Pharmacy, the Core has an Applied Biosystems4000 Q-trap LC MS/MS system with ESI, APCI and nanospray sourcescoupled with a Shimadzu LC-20AD HPLC system, ThermoFinnigan LCQion trap mass spectrometer with ESI, APCI and microspray interfacescombined with a Michrom Magic 2002 HPLC system, a ThermoFinniganTrace MS GC-quadropole with EI positive, negative CI and selected ionmonitoring (SIM), an ABI Voyager-DE Pro MALDI-TOF and a Bio-radBioplex 200 fluorescent microbead array system.174 Microscopy and Imaging Facility,Cornell UniversityR. Williams, C. Bayles, J. Dela Cruz, M. Riccio,R. Doran, W. ZipfelCornell University, Ithaca, NY, United StatesThe Microscopy and Imaging Facility of the Cornell University LifeSciences Core Laboratories Center (CLC) provides an array of sharedresearch resources and services relating to optical microscopy,fluorescence, and whole animal imaging. The mission of the facility isto provide cutting edge technologies and high quality services that willsignificantly contribute to life sciences research, training and educationprograms.175 Utilization of Flow Cytometry inPersonalized MedicineP. LopezNew York University, New York, NY, United StatesFlow cytometry is a multiparametric data-gathering tool with anenormous, well-established array of applications for intact cells,organelles, functionalized beads, lysed cell contents or supernatants.Multiparametric analysis using state-of-the-art cytometers has thepotential to yield a distribution of cellular phenotypes with highdimensionality (up to 20 parameters per cell simultaneously) specificto an individual disease state or system-wide perturbation. Purifiedpopulations of atypical and/or cells representative of the disease statecan be collected as the first step towards biomolecular characterization.Flow cytometers may be outfitted with multiwell-plate accessories toprovide single-cell capture of specified cells with correlation to eachcell’s individual phenotype (indexed sorting), or rapid sampling frommicrotiter plates (plug-flow). An exciting new cytometric innovationmay allow determining the full signature of biomarkers from individualcells . The massively multiparametric mass cytometer analyzer makesuse of the high sensitivity and resolution of mass spectroscopy torecord the elemental composition as well as metal-tagged biomarkerdistribution of individual cells at a rate of up to 1000 cells per second.176 Cell Imaging and Analysis Network(CIAN) - Multi-Platform Resourcesand Servicesof six scientific platforms: Cell Imaging (confocal and fluorescencemicroscopy; walk-up), Proteomics (2-D, DiGE and fluorescent proteinanalysis; walk-up), Automation and High throughput screening(Pinning robot and liquid handler; full service), Protein expression andantibody production (in collaboration with local animal facilities; fullservice), Genomics (real-time PCR; walk-up), and Data storage/analysis(cluster, server and workstations). Users get in-depth consultationfor proposed projects, and can obtain training in any of the walk-upaspects of the facility, or take advantage of the full-service platforms.CIAN is designed to facilitate training, enhance interactions, as well asshare and maintain resources and expertise.177 Danforth Center: Proteomics & MassSpectrometry Core FacilityL. Hicks, S. Alvarez, B. Zhang, Z. Liu, H. Wang,J. FazlicDanforth Center, St. Louis, MO, United StatesThe Proteomics & Mass Spectrometry Facility at the Donald DanforthPlant Science Center (http://www.danforthcenter.org/pmsf/) isequipped with state-of-the-art technologies for the detailed study ofa wide range of biomolecules. The facility provides both full- and selfservicecapabilities to both internal and external clients at competitiverates. The facility offers fast, high quality specialized analytical servicesincluding: protein extractions, liquid chromatographic separations; highresolution 1D/2D gel electrophoresis; gel image analysis and proteinexpression analysis; high-throughput protein spot excision; in-solutionand in-gel protein digestion; high-throughput protein identification;accurate protein molecular weight analysis; protein covalent/noncovalentcomplex analysis; biomolecule interactions (surface plasmonresonance); small molecule separation/structure determination; andprotein post-translational modification analysis. Major instrumentationincludes: LTQ Orbitrap Velos (Thermo Scientific), QSTAR XL Q-TOF,two 4000QTRAPs, two 6520 Q-TOFs (Agilent), 5975C GC-MS(Agilent), TriVersa NanoMate (Advion), Chip Cube (Agilent), two 1200HPLCs (Agilent), 3 nanoflow HPLCs (LC Packings/Eksigent), SystemGold HPLC (Beckman Coulter), two Shimadzu HPLCs (Shimadzu),UPLC (Waters), Biacore2000,3100 OFFGEL fractionator (Agilent), 1Dand high resolution2D gel electrophoresis systems (BioRad/AmershamBiosciences), Typhoon 9410 (Amersham Biosciences), GelPix (Genetix),and MultiProbe II (Perkin-Elmer). Protein intact mass, identification andcharacterization are a few of the many applications that the facilityperforms. For proteomics applications,two Q-TOFs andthe LTQOrbitrap Velos instruments are well-suited for analyzing both smallpeptides and large proteins. The LTQ Orbitrap Velos can be set upwith the TriVersa to automate direct infusion of samples to performexact mass measurements for molecular formula determination orfacilitate targeted analysis of modifications. The LTQ Orbitrap Velosand the 6520 Q-TOF are also used for online LC-based quantitativeproteomics (iTRAQ and label-free). The 4000 QTRAP systems serveas powerful instruments for targetedmetabolite analyses (e.g. planthormones, pABA, etc.), and the GCMS and one LC-QTOF are used formetabolomics profiling initiatives.Poster AbstractsE. Küster-Schöck, J. Lacoste, G. Lesage,S. Bunnell, H. HanMcGill University, Montreal, QC, CanadaThe Cell Imaging and Analysis Network (CIAN) provides servicesand tools to researchers in the field of cell biology from within oroutside Montreal’s McGill University community. CIAN is composedABRF 2011 — Technologies to Enable Personalized Medicine • 81