Conference Program - ABRF 2011 - Association of Biomolecular ...

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Satellite Educational Workshop Sponsors(SW1) Protein Purification for Mass Spectrometry(SW2) An Introduction to Metabolomics(SW3) Next Generation Sequencing Considerations for Core ProfessionalsSatellite EducationalWorkshops(SW4) Lean Management in Core Facilities28 • ABRF 2011 — Technologies to Enable Personalized Medicine
Satellite Educational WorkshopsAll Satellite Educational Workshops will be held on Saturday, February 19 at the JW Marriott San Antonio Hill Country Resort.Attendees must register separately for all Satellite Educational Workshops. To register, please visit the Registration Desk on Level 2.For further information and for individual workshop schedules, please review the proceeding pages.SW1: Protein Purification for Mass Spectrometry8:00 am – 4:00 pm Level 2, Grand Oaks Ballroom ROrganizers & Presenters: Robert Carnahan, and W. Hayes McDonald, Vanderbilt University Medical CenterWorkshop Description: The workshop is intended as a practical guide for investigators wishing to purify proteins and proteincomplexes for mass spectrometry based analysis. It should be useful for individuals wishing to perform such purifications as well asfor mass spectrometrists who will be analyzing these types of samples. We will explore various considerations and possibilities indesigning and executing protein purifications intended for mass spectrometry and then focus on antibody-based affinity purifications.While there will not be time to take workshop participants through all of the hands-on aspects, our intent is to guide them throughdesigning and troubleshooting the various stages of the process. We will then utilize data generated from an actual hands-on course,and allow the participants to see and interpret some real data that they have “watched” being generated.Workshop Agenda7:00 am – 6:00 pm Registration Open — Level 2, Ballroom Level Registration Desk7:00 am – 8:00 am Continental Breakfast — Level 2, Grand Oaks Ballroom P/Q8:00 am – 9:00 am Affinity Purification for Mass SpectrometryPresenter: Robert Carnahan, Vanderbilt University Medical Center9:00 am – 10:00 am Mass Spectrometry Analysis of Protein ComplexesPresenter: W. Hayes McDonald, Vanderbilt University Medical Center10:00 am – 10:30 am Morning Refreshment Break — Level 2, Grand Oaks Ballroom P/Q10:30 am – 12:00 pm Virtual “Hands-On” WorkshopPresenters: W. Hayes McDonald and Robert Carnahan, Vanderbilt University Medical CenterReagent generation and QCBiological OptimizationSample Preparation and ProcessingData Analysis12:00 pm – 1:00 pm Lunch — Level 2, Grand Oaks Ballroom P/Q1:00 pm – 2:30 pm Exploration of Experimental Design Considerations and their ImplicationsPresenters: W. Hayes McDonald and Robert Carnahan, Vanderbilt University Medical Center2:30 pm – 3:00 pm Afternoon Refreshment Break — Level 2, Grand Oaks Ballroom P/Q3:00 pm – 4:00 pm Alternative ApproachesPresenters: W. Hayes McDonald and Robert Carnahan, Vanderbilt University Medical CenterSatellite EducationalWorkshopsABRF 2011 — Technologies to Enable Personalized Medicine • 29
Page 7 and 8: Meeting SponsorsThe Association of
Page 9 and 10: Registration ServicesRegistration S
Page 12 and 13: Presenter InformationPresenter Info
Page 14 and 15: AwardsABRF Annual Award for Outstan
Page 16 and 17: ABRF Robert A. Welch Outstanding Re
Page 18 and 19: Program-at-a-GlanceProgram-at-a-Gla
Page 20 and 21: Sunday, February 20 (Continued)(S1-
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Page 32 and 33: SW2: An Introduction to Metabolomic
Page 34 and 35: SW4: Lean Management in Core Facili
Page 36 and 37: Plenary Session AbstractsPlenary Se
Page 38 and 39: Scientific Session AbstractsScienti
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Page 62 and 63: Poster Session Abstracts**ABRF Post
Page 64 and 65: Poster Abstractsglobal gene express
Page 66 and 67: Poster Abstractsdiscussions of cont
Page 68 and 69: Poster Abstractspossible applicatio
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Page 73 and 74: distribution. Additionally, we show
Page 75 and 76: Illumina Genome Analyzer. PicoPlex
Page 77 and 78: uniformity, reproducibility of enri
Page 79 and 80: kits for Tempus TM and PAXgene® st
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165 Benchmarking miRNA ExpressionLe
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Phoenix crystallography robot and a
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Laboratory is located next door and
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data of a Thermo Orbitrap instrumen
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**197 Multi-Functional Superparamag
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pattern of N-linked glycans in form
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advantage of using two separate 15
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allowed absolute quantitation. Comp
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levels between the animal groups. A
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MuDPIT, and off-gel electrophoresis
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237 Multi-Site Assessment of Proteo
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with good limits of quantification
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249 Rapid Monoclonal Antibody Glyca
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OOng, J. - 117PPatel, S. - 169Peake
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AnaSpec, Eurogentec Group Booth 417
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FASEB MARC Program Booth 4169650 Ro
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IntegenX, Inc. Booth 2015720 Stoner
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Nonlinear Dynamics Booth 1052530 Me
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Roche Applied Science Booth 5009115
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Exhibit Hall FloorplanGrand Oaks Ba
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Exhibitor List in Booth OrderExhibi
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This workshop will present ways to
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Grand Oaks Ballroom,Rooms P&Q, Leve
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NotesNotesABRF 2011 — Technologie
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Tuesday, February 22 — 12:00 pm -
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MARCH 16-20, 2012 • DISNEY’S CO
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