Vendor Presentations — ContinuedMonday, February 21 — 12:00 pm – 1:30 pm (Continued)Vendor PresentationsGrand Oaks Ballroom,Rooms N&O, Level 2Exhibit Booth: 601From Discovery to TranslationalGenomics with Life Technologies’Comprehensive Sequencing SolutionsLife Technologies <strong>of</strong>fers the most comprehensive and complementaryportfolio <strong>of</strong> research sequencing solutions — fromthe market leading Capillary Electrophoresis solutions, throughthe recently launched Ion Torrent Personal Genome Machine,(PGM) to SOLiD TM systems — with the 5500 series SOLiD TM systemsbecoming available soon. The re-engineered 5500 seriesSOLiD TM system generates up to 30 gigabases <strong>of</strong> mapped dataper day and can be used in a myriad <strong>of</strong> research applications— including complete cancer genome re-sequencing, single celltranscriptome analysis, interrogation <strong>of</strong> methylation status, andde novo sequencing. The increasing throughput <strong>of</strong> the evolvingSOLiD TM platform and other next generation sequencingtechnologies now makes it possible to sequence a whole humangenome. As additional human genomes are sequenced, newbiological insights are obtained, and we begin to truly appreciatethe critical need for sequencing accuracy. Consensus accuracycan overcome some inherent error rates, however a flooris ultimately reached, thereby limiting identification <strong>of</strong> low frequencysomatic mutations or SNV’s when using low coveragetechniques for GWAS based projects. A new chemistry incorporatingerror-correction codes has been developed that enablesthe SOLiD TM system to achieve 99.99% accuracy within a singlesequencing read. This, and other technological improvements,will be discussed in the context <strong>of</strong> the emerging “TranslationalGenomics” era that we have recently entered.PostLight Sequencing with Semiconductor ChipsIon Torrent has invented the first device—a new semiconductorchip—capable <strong>of</strong> directly translating chemical signals intodigital information. The first application <strong>of</strong> this technology is sequencingDNA. The device leverages decades <strong>of</strong> semiconductortechnology advances, and in just a few years has brought theentire design, fabrication and supply chain infrastructure <strong>of</strong> thatindustry—a trillion dollar investment—to bear on the challenge<strong>of</strong> sequencing. The result is Ion semiconductor sequencing, thefirst commercial sequencing technologythat does not use light,and as a result delivers unprecedented speed, scalability andlow cost.Next Generation qPCR solutions from Life TechnologiesIn 2010, Life Technologies TM released the Applied Biosystems TMViia TM 7 Real-Time PCR System. This seventh generation systemcombines advances in optical and thermal cycling technologyto provide one the most powerful and flexible Real-Time PCRinstruments available on the market today. After a brief review<strong>of</strong> the Viia TM 7 Real-Time PCR System, we will explore the nextgeneration <strong>of</strong> qPCR systems.The OpenArray® Real-Time PCR platform employs 3072 wellreaction plates to increase throughput and to lower costs forqPCR. The throughput and cost efficiencies <strong>of</strong> the OpenArray®Real-Time PCR platform are enabling applications such as Next-Gen Sequencing library quantitation by digital PCR, gene expressionpathway analysis, high-throughput genotyping studies,and miRNA pr<strong>of</strong>iling. The OpenArray® Real-Time PCR platformdelivers TaqMan® sensitivity and specificity in miRNA pr<strong>of</strong>ilingwhile achieving cost savings, comparable to microarray systems.The throughput for miRNA pr<strong>of</strong>iling is up to 9 samples completedin a 2.5 hour run, and up to 36 samples per day.The next generation <strong>of</strong> Life Technologies’ TM qPCR systems alsoincludes a novel technology platform based upon a continuousflow liquid-bridge manifold. This will enable high throughputGenotyping and Gene Expression applications. The Stokes Bionanodroplet qPCR technology will be reviewed during this talk.Alyssum Room, Level 3Exhibit Booth: 500Amplicon Sequencing with GS FLX andGS Junior SystemsAccess Array TM System High-Throughput ResequencingSpeaker: Steven H<strong>of</strong>fman, Fluidigm CorporationFlexible Next-Generation Sequencing Approaches Enabledby the GS FLX and GS Junior SystemsSpeaker: Bruce Taillon, Ph.D., 454 Life SciencesFeaturing GS FLX Titanium series chemistry, the Genome SequencerFLX and GS Junior Systems <strong>of</strong>fer a powerful combination<strong>of</strong> long sequencing reads (400 to 500 base pairs) and dedicatedGS Amplicon Variant Analyzer (AVA) s<strong>of</strong>tware, enablingthe sequencing, alignment, and analysis <strong>of</strong> amplicons against areference sequence.122 • <strong>ABRF</strong> <strong>2011</strong> — Technologies to Enable Personalized Medicine
Grand Oaks Ballroom,Rooms P&Q, Level 2Exhibit Booth: 505Controlling Experimental VariablesSpeaker: Jim Walters, Ph.D., Principal Investigator, AnalyticalResearch Department, Sigma Life ScienceThe enormous amount <strong>of</strong> data generated from complex proteomicanalyses with mass spectrometry is <strong>of</strong>ten overwhelming;requiring iterative analysis and optimization <strong>of</strong> a number <strong>of</strong> variables.To counteract the effects <strong>of</strong> potentially false data, a number<strong>of</strong> defined protein and peptide standards have been developedto allow for universal performance comparisons acrossplatforms and between labs. We will highlight our efforts in thedevelopment, commercialization, and utilization <strong>of</strong> proteomicsstandards. We will discuss our newly developed standards whichinclude both phosphopeptide standards as well as a syntheticprotein designed to fully assess standard LC-MS platforms.Begonia Room, Level 3Exhibit Booth: 108Recent Developments in WatersProteomics TechnologySpeaker: Martha Stapels, Principle Research Scientist,WatersWe will be discussing advances in ETD, Ion Mobility andseparations. Also featured will be a presentation by J. WillThompson from Duke University: “Absolute Quantitation Using2DLC-MSE and Species-Specific Correction to InvestigateChlamydia trachomatis Developmental Forms.”Monday, February 21 — 7:30 pm – 9:00 pmAlyssum Room, Level 3Exhibit Booth: 500Reviews <strong>of</strong> SeqCap EZ Exome v2.0Vendor Presentations<strong>ABRF</strong> <strong>2011</strong> — Technologies to Enable Personalized Medicine • 123
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