SYNERGISTIC EFFECTS OF MICROBIAL ANTAGONISTS AND ...

vtz.. ptroleum ether. chloroform, acetone and ethanol were obta~ned using soxhletapparatus Three concentrattons of the above extracts (2 5. 5 & 10 %) of each of fivespecles were tested for the~r anttfungal actl\~t\ agatnst X solonr in vrtro2.3. Preparation of extracts:2.3. I. Preparation of extra& with solvents: For the screening of plants. extracts werepepred ~tth d~llcrenr orgunlr. sol\ents lh~s IS kuusct ofthe fact that \ar~ed typesol' suhslancr% extracted In d~llkrcnt solrents m~ght have potenttal ~nh~b~to~suhslanc~~ against palhogcns (Coop and Ciunns. 19'45)SoI\cnls uscd tor the cttrarllon rrcrc4 I'etrol~wm ether I o xparatc rvates. sterol, strro~ds etc (60-80 Y).' C'hlorotormAc~lonelo x-parete aihalotds 160-h2 OC')I o sc-parate reslns ( 55 i-56 5 -i')4 1:thanol 1 o separate rcwns and lo\+ plar gl! cosldcrs ( 70 "C)2.3.2. Edtadim Prorrs5: Ihr. Ica\cs rrcrc rtashed In d~sttllal Haler and dr~ed at.37 "C' I! mar then pr)urk.red and taken tbr sttractton Ihe r\tractlon uns done Insoxhlet applratus Afkcr pch~ng the r ~al tr~th cotton. 15 g of the test matertal waslill~d lthc rr-spn.tt\c wlren! rrhlch &\a\ und Ibr the extractton uas 1*1llcul In thehottom 11mh Ihe apparatus has a condmscr In rrhlch the lapour artslng rn the tlashpas- h\ Ihc. SI& ~ u Into k the condcn.rcr I krc tt conden.ses and drlps into the ho4\01' thc cktrwior, n prtn~on pisslng Into thc s\ptwn luk s~phons orer and runs tnto theIlasA, thus cmptr mg hc hhJ\ t k e\twtor I'h~s alternnt~on of lill~ng and ernpt? in$the bud) ofthe cxuac-tor \rmt on wn~tnuousl\ h\ rrh~ch ItK. %~lublc mantr Jtswl\.edfrom thr lrsl malcttal In he cxmmr rernn~nd In the Ilash. uh~le the .wl\snt *asrcpealnll) rola~~ltrrd I'h~s prxc'u; \\as repatrcf 14 to I5 trmes unt~l~qu~d In ther~phm tubc hecame ~wlourlrss (t8skc and Vuon. 19971