SYNERGISTIC EFFECTS OF MICROBIAL ANTAGONISTS AND ...

3.2.3. PoLFoned Plate Technlquc The mycellal growth of R soianr was measured bypotsoned plate techntque. Afkr the ster~lizat~on of' peutplates (9 cm dta ). PDArnedtum. cork borer and other glass wares in an autoclave at 121.5 "C for 15 mtn wtth15 IWsq tnch pressure, the prepared culture filterates of' the b~ocontrol agents andphgioextracts three dtli'crent concentrattons vtz. 2 5%. 5% and 10% were added tothe warm I'iIA In three Petrt plates Then the plates were tnoculated by plac~ng 9 mm(d~a) dtsc cut from the gro\rtng ttp 01- 5 days old culture of' R soian~ All thts wascaned out under the stertle condttton of' lamtnar flow chamber PDA wtthout theextract mrxl as controlI he control and treated cultures were rnalnta~ned tntr~pl~ates I'hr ~noculatrd plates were waled wtth parafilm and tncubated in the~ncuhator at ?Us0 ? "C'urAm along thc radtal IIWOn the 5 "' di~! ol' tncuhat~on. the measurement (em) brascil thc m\cel~al gro\rIh tn thc ptrrplates I he percentage ofm\crlwl groulh tnhrhlt~on Has calculated h mlng thc tbrmula of Vtncent ( 1927)C'- II , ----I. * 100c'H'krc.I -: Inhth~tlon of m\ecI~aI gro~thc' -.. 1)lamrtcr gro\\rh In control4. ('E1.1. \I Ai.1. DE(;HADISti ESX1'3IE ASSAI S:4. I. EuMcilm Procedwrc l.or p~~~nol)ttc sw! mc prndwtlon the pathogen wisgrown tn crapck's broth. supplemnt~d ~tth Frttn a.r carh~n source replac~ngsuurosc S~m~larf\. for ccilulol~tr enq mts mtcrocp stalltne ccllulos+. and carhou!mh) 1 ccllulosc w e usrd