SYNERGISTIC EFFECTS OF MICROBIAL ANTAGONISTS AND ...

5.5. Assrssmmr of the eflcacy of mfenl concmlrntfons of the an1agonLFIJ andphv oudracls:One kg of soybean seeds (COZ) was soaked for 12 h In 400 rnl of \ratercontnlnlng the talc hased product of antagon~sts adlusted to the respective dosage-100e/ Kg seeds F.?tcess water was dralned off and treated seeds were Incubated in thedark for 24 h aner uh~ch they were soun In control. sterllc d~stilled \rater was usedInstead of the prnduct Pot culture espcrlmrnt was la~d out In Cornplctel! Random~sedIk'slgn (C'RL)) w~th thrt~ repltcat~ons for cach treatment 30 seeds were soun In eachpnt'I'he voung lea\c7; of 30-&?.-old plants _een~nated from the abve seeds were~ncrulated wth R .cdunt h spra?.lng on thc Ica\es aRer the? Hpre Inlured with thehclp of surgtcal nwJles Intrulat~cln of H .ro/i~nt\$as done In the earl! hours 6 00-7 30 AM Conlrnl plants nerc spra\rxi ~ ~ the t wm h \olurne ofstenlc d~st~lled naterAll Ihe plants werc ~mmedlatel) co\ered ulth pl!thene hags sprinkled \rfth steriled~sltllrd uotrr on the Inner side so as to malntatn h~ghum~d~t\ and kepi und~stumed(br 24 h Altcr 24 h, plant extract mas spra\cd (31 IIAS) on the leaves The laveswrrc cnllcc~cd on IO* da! alter 1st spnl) of ph?tnextract for thc rst~rnatlon of' thevarious paramclen 'I'k kmnd spra! u.ns glvcn 15 da\.s (46 DAS) atter the 1st spra!lhen the Icavn ncrc collertcd on 10'~ da! alter 2" spra! and trcrc used forcst lmaltonIN I'II"0 Sl'l DIM:d EXPERIME.%lS WERE DESIG.KED .4S FOLLOWS:I Control (; nar plants mtsal l'm the srvds waLed w~th dlst~llcd Hater f'or 12 hovan~ghl