SYNERGISTIC EFFECTS OF MICROBIAL ANTAGONISTS AND ...

4.5. Esrlmarlon of CeNobime: 'The amount of reduclng sugars released from thecellohlase was used to assay the enzyme.Rapents:1 Sdlum acetate-acetic ac~d butrer, pH 5 82 5 mM cellohloseMetbod : 1 5 ml of the huller. 2 5 ml of5 mM crllob~ose and 1 ml of the enzymesource was taken m n tcst tuk and tncuhated at 30°C lor 2 h7-he reactlon waslennlnated h placing the test tuhe In a bolllng water bath for 10 mln. The amount ofglucosr. llheratcd h\ the ennme uslng 1)NS reagent was measured at 575 nm InSystron~s Spcxuophotomter (jlucax was used as standards 1.4' 171s-1'1 1)iKS:5. I. .See& used for Ihr pot u'pcrimenls: I'hc C'O: \ arlct! of (; mar h4r.n (I.)\\.asun-d Tor the pot1r.d m t.n,o studlea I'hc c\perlmcnts were carrlcd out In the School of1.1fe kwnws. PnMllc.krn Irn~\crsln I'hc pots \rere prepared according to theswndnrd prcx.cdur~.s Ir~pllcstm \\r.rc rnalntslncd tbr all treatments5.2. Prrpararlnn of ralr bawd formulutian of mlctobial antagonists: Talc hasedSorrnulatlom n i I' fluc~rc.rr.c.nstriiln5 \\ere prcparrd h the method of \'~dh\axloranand hlu~harnllan ( I*) P Rrrr,rrsccnr stralris were mult~plled In K1ng.s B broth Theh,th was autcxla\rrd st I 4 kgicni' fi)r 20 rntli h Itmpful ot' the hactcrla \\as~nc*.ulalr+d Inlo the txuth and ~nculwt~d In a rotaton shaker a1 I50 rpni litr 48 h stroom tcrnpcraiurc (2nd 4') 1hc hucter~al populauon In the broth uas aausted toYx I 04cfdmlIn the cax 01'7' kacrunlrm Icmpiul of fungal antagonist itas scraped out and~mulr~cd In che hmth and ~ncuhotd In the rotaton shaker at 150 rpm for 48 h Thefungrl m~crohul anmgon~si populatmn In thc both \\as adlusted to 6s10d cfdml